Evidence of gene introgression in apple using RAPD markers |
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| Authors: | Richard E Durham Schuyler S Korban |
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| Institution: | (1) Department of Horticulture, 310 PABL, University of Illinois, 1201 W. Gregory, 61801 Urbana, IL, USA;(2) Present address: Department of Plant and Soil Science, Texas Tech University, Boc 42122, 79409 Lubbock, TX, USA;(3) Texas Agricultural Experiment Station, Texas A&M University, Boc 42122, 79409 Lubbock, TX, USA |
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| Abstract: | Summary A genomic remnant of Malus floribunda clone 821 introgressed into the cultivated apple M. x domestica Borkh. was identified using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). Using a set of 59 oligonucleotide decamer primers, polymorphic DNA markers were identified among three pooled DNA samples. Based on the presence or absence of bands among bulked apple scab-resistant selections and cultivars, bulked scab-susceptible cultivars, and a M. floribunda clone 821 sample, one primer, A 15, identified amplified fragments in the scab-resistant bulked sample that was also unique to the M. floribunda clone 821. The unique band from M. floribunda clone 821 was amplified in four out of 17 scab-resistant selections/cultivars. This RAPD, designated OA15900, identifies an introgressed fragment that has as yet no known function. |
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| Keywords: | apple Malus gene introgression RAPDs PCR DNA markers Venturia inaequalis apple scab resistance |
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