| 魔芋高效再生体系的建立与优化 |
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| 引用本文： | 孙继华,杨超,刘菊华,张静.魔芋高效再生体系的建立与优化[J].热带作物学报,2012,33(1):84-88. |
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| 作者姓名： | 孙继华 杨超 刘菊华 张静 |
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| 作者单位： | 1.农业部热带作物生物技术重点开放实验室/中国热带农业科学院热带生物技术研究所；
2.海南省嘉积中学
3.中国热带农业科学院科技信息研究所 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项 |
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| 摘 要： | 研究不同激素及浓度配比对魔芋愈伤组织、不定芽和根诱导的影响。结果表明，诱导愈伤组织的最佳激素组合为ＭＳ＋６－ＢＡ０.5 ｍｇ／Ｌ＋ＮＡＡ ０．５ ｍｇ／Ｌ，其诱导率为８２％；诱导不定芽分化的最佳配方为ＭＳ＋６－ＢＡ １．５ ｍｇ／Ｌ＋ＮＡＡ ０．５ ｍｇ／Ｌ，其分化率为４９０％；诱导生根的最佳激素组合配方为ＭＳ＋ＮＡＡ ０．５ ｍｇ／Ｌ＋６－ＢＡ １．０ ｍｇ／Ｌ，其生根率为９０％。
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| 关 键 词： | 魔芋 组织培养 高效再生 |
Establishment of an Efficient Regeneration System of Amorphophallus konijac |
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| SUN Jihua
,
YANG Chao
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LIU Juhua
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ZHANG Jing.Establishment of an Efficient Regeneration System of Amorphophallus konijac[J].Chinese Journal of Tropical Crops,2012,33(1):84-88. |
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| Authors: | SUN Jihua YANG Chao LIU Juhua ZHANG Jing |
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| Institution: | 1.Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences；
2.Hainan Jiaji Middle School
3.Institute of Scientific and Technical Information, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | Aimed at establishing an efficient regeneration system for Amorphophallus konijac and providing a theoretical foundation for large-scale production in factories,the effects of different phytohormones on callus,bud and root were investigated.The results indicated that the optimum culture medium of callus initiation was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L and the regeneration rate was 82%.The optimum culture medium of bud differentiation was MS+6-BA 1.5 mg/L+NAA 0.5 mg/L and the regeneration rate was 490%.The optimum culture medium of rooting was MS+NAA 0.5 mg/L+6-BA 1.0 mg/L and the rooting frequency was 90%. |
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| Keywords: | Amorphophallus konijac Tissue culture Efficient regeneration |
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