| 水稻蛋白OsOlel在大肠杆菌中的诱导表达 |
| |
| 引用本文： | 姚清国,李小兵,韩爱云.水稻蛋白OsOlel在大肠杆菌中的诱导表达[J].安徽农业科学,2011(19):11399-11400. |
| |
| 作者姓名： | 姚清国 李小兵 韩爱云 |
| |
| 作者单位： | 石家庄学院化工学院; |
| |
| 基金项目： | 石家庄市科学技术研究与发展课题(10120803); 石家庄学院科研启动基金(2007012) |
| |
| 摘 要： | 目的]在大肠杆菌中诱导表达水稻蛋白OsOle1。方法]RT-PCR克隆水稻基因OsOle1,构建原核表达载体pGEX-2T-OsOle1-GST,在大肠杆菌BL21中诱导表达该蛋白,并采用GST凝胶亲和层析进行纯化。结果]水稻蛋白OsOle1具有典型的Olee1蛋白家族的保守域,N端1～24位氨基酸为预测的信号肽序列。成功地将水稻蛋白OsOle1在大肠杆菌中进行了诱导表达,并用GST亲和层析进行了纯化。结论]该研究为以后进一步研究水稻蛋白OsOle1的生理功能奠定了基础。
|
| 关 键 词： | 水稻 OsOlel 诱导表达 亲和层析 |
Induced Expression of Rice Protein OsOle1 in Escherichia coli |
| |
| YAO Qing-guo et al.Induced Expression of Rice Protein OsOle1 in Escherichia coli[J].Journal of Anhui Agricultural Sciences,2011(19):11399-11400. |
| |
| Authors: | YAO Qing-guo |
| |
| Institution: | YAO Qing-guo et al(College of Chemical Engineering,Shijiazhuang University,Shijiazhuang,Hebei 050035) |
| |
| Abstract: | Objective]The research aimed to induce the expression of rice protein OsOle1 in Escherichia coli.Method]RT-PCR was used to clone OsOle1 in the rice,and the prokaryotic expression vector pGEX-2T-OsOle1-GST was constructed.The protein was induced expressing in Escherichia coli BL21,and GST gel affinity chromatography was used to purify.Result]The rice protein OsOle1 had the typical conserved domain of Olee1 protein family.The amino acids at 1-24 sites in the N terminal were the predicted signal peptide seq... |
| |
| Keywords: | Rice OsOle1 Induced expression Affinity chromatography |
| 本文献已被 CNKI 维普 等数据库收录！ |
