| 重叠区扩增法合成抗菌肽B基因 |
| |
| 引用本文： | 桑春果,张开春,张军科.重叠区扩增法合成抗菌肽B基因[J].西北农林科技大学学报(社会科学版),2002,30(1):65-67. |
| |
| 作者姓名： | 桑春果 张开春 张军科 |
| |
| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西,杨陵,712100
2. 北京市农林科学院,林果所,北京,100093 |
| |
| 基金项目： | 北京市科委合同项目 ( 85 5 130 40 0 ) |
| |
| 摘 要： | 人工设计并合成了抗菌肽 B基因的 4个寡聚核苷酸片段 ,通过重叠区扩增法 ,扩增出了相当于抗菌肽基因全长的寡聚核苷酸片段。经克隆测序 ,证明成功地实现了抗菌肽基因的人工合成、拼接和克隆。
|
| 关 键 词： | 重叠区扩增法 抗菌肽 基因合成 基因克隆 |
| 文章编号： | 1000-2782(2002)01-0065-03 |
| 收稿时间： | 2001/9/17 0:00:00 |
| 修稿时间： | 2001年9月17日 |
Synthesis and cloning of antibacterial peptide B (cecropin B) gene by overlap extension |
| |
| YANG Ya-ti,ZHANG Yi-ping,ZHANG Xing-fu.Synthesis and cloning of antibacterial peptide B (cecropin B) gene by overlap extension[J].Journal of Northwest Sci-Tech Univ of Agr and,2002,30(1):65-67. |
| |
| Authors: | YANG Ya-ti ZHANG Yi-ping ZHANG Xing-fu |
| |
| Institution: | 1 (1 College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China; 2 Institute of Pomology and Forestry,Beijing Academy of Agriculture and Forestry,Beiji |
| |
| Abstract: | Designed and synthesised 4 fragments of antibacterial peptide B gene,then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension (gene SOEing).The interested fragment was cloned and sequenced and showed a good result as expected. |
| |
| Keywords: | gene splicing by overlap extension antibacterial peptide gene synthesis gene cloning |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农林科技大学学报(社会科学版)》浏览原始摘要信息 |
| 点击此处可从《西北农林科技大学学报(社会科学版)》下载免费的PDF全文 |
