| 携带抗黄矮病基因染色体的分离 |
| |
| 引用本文： | 何聪芬,钱江.携带抗黄矮病基因染色体的分离[J].作物学报,1999,25(3):273-278. |
| |
| 作者姓名： | 何聪芬 钱江 |
| |
| 作者单位： | 中国农业科学院作物育种栽培研究所作物遗传育种农业部重点实验室，北京，100081 |
| |
| 摘 要： | 抗黄矮病小麦一中间堰麦草异附加系Z2 (2n=44)携带一对完整的中间僵麦草染色体，用Z2作母本与普通小麦品种中8601杂交，获得杂种F1(2n=43=21Ⅱ+1I)。利用激光显微切割技术将F1花粉母细胞减数分裂中期I及后期I的呈单价体的中间僵麦草染色体分离出来，经去蛋白、Sau3AI酶切后，进行PCR体外扩增。结果表明利用激光显微切割可分离
|
| 关 键 词： | 小麦 染色体激光显微切割 染色体DNA PCR扩增 |
| 收稿时间： | 1998-04-13 |
| 修稿时间： | 1998-09-21 |
Isolation of the Single Chromosome with BYDV Resistant Gene |
| |
| He Congfen Ma Youzhi Xin Zhiyong Xu Qiongfang Qian Hong.Isolation of the Single Chromosome with BYDV Resistant Gene[J].Acta Agronomica Sinica,1999,25(3):273-278. |
| |
| Authors: | He Congfen Ma Youzhi Xin Zhiyong Xu Qiongfang Qian Hong |
| |
| Institution: | Institute of Crop Breeding and Cultivation, CARS, Beijing 100081 |
| |
| Abstract: | Wheat/Th.intermedium alien addition line Z2 carrying one pair of Th.intermedium
chromosomes showed good resistance to barley yellow dwarf virus (BYDV) and was used as female parent to cross with wheat variety Zhong 8601. The chromosomal construction in F1 was 2n=43=21Ⅱ+1I.The univalent chromosome |
| |
| Keywords: | Wheat Chromosome microdissection Chromosome DNA PCR |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
