| HPLC法测定异株荨麻茎叶中绿原酸和黄酮类化合物 |
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| 引用本文： | 何斌. HPLC法测定异株荨麻茎叶中绿原酸和黄酮类化合物[J]. 湖北农业科学, 2012, 51(10): 2101-2103 |
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| 作者姓名： | 何斌 |
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| 作者单位： | 毕节学院环境与生命科学系,贵州毕节,551700 |
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| 基金项目： | 国家科技部星火计划项目“生态建设与经济林果栽培技术与科技示范”;贵州省教育厅自然科学研究项目(黔教科2010075) |
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| 摘 要： | 建立了一种可同时测定异株荨麻(Urtica dioica L.)茎叶中绿原酸和黄酮类化合物的高效液相色谱法,色谱柱为钻石C18柱,流动相为甲醇和0.4％磷酸混合液(体积比为55/45),检测波长355 nm,柱温25℃,流速1.0 mL/min.结果表明,本法简便、快速、有效、准确、重现性好,可用于异株荨麻茎叶中绿原酸和黄酮类成分的定量分析.
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| 关 键 词： | 异株荨麻(Urtica dioica L.) 绿原酸 芦丁 槲皮素 高效液相色谱 |
Determination of Chlorogenic Acid and Flavonoids in Stem and Leaf of Urtica dioica by HPLC |
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| HE Bin. Determination of Chlorogenic Acid and Flavonoids in Stem and Leaf of Urtica dioica by HPLC[J]. Hubei Agricultural Sciences, 2012, 51(10): 2101-2103 |
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| Authors: | HE Bin |
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| Affiliation: | HE Bin(Department of Environment and Life Science,Bijie University,Bijie 551700,Guizhou,China) |
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| Abstract: | HPLC method was established for determination of chlorogenic acid and flavonoids in the sample of Urtica dioica L.The sample was separated by Diamonsil C18 at 25 ℃ with the mobile phase of methanol and 0.4% phosphate acid(V/V=55/45),and detected at 355 nm.The flow rate was 1.0 mL/min.In a conclusion,the method is simple,effective,feasible and reproducible.It can be used to determine chlorogenic acid and flavonoids in U.dioica. |
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| Keywords: | Urtica dioica L. chlorogenic acid rutin quercitrin HPLC |
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