| 影响农杆菌介导的小麦遗传转化条件的研究 |
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| 引用本文： | 贺杰,王伟,胡海燕,赵俊杰,校现周.影响农杆菌介导的小麦遗传转化条件的研究[J].种子,2010,29(5). |
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| 作者姓名： | 贺杰 王伟 胡海燕 赵俊杰 校现周 |
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| 作者单位： | 1. 河南科技学院,河南,新乡,453003
2. 中国热带农业科学院橡胶研究所,海南,儋州,571737 |
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| 基金项目： | 河南科技学院重点科研项目资助基金，河南省科技厅科技攻关项目 |
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| 摘 要： | 利用小麦成熟胚愈伤组织的高效再生体系,对影响农杆菌介导的小麦遗传转化条件:预培养时间、共培养时间、抗生素(Kan)筛选压、头抱霉素浓度、菌液浓度和侵染时间等进行了研究。最后确立的转化条件为:预培养时间为1～2d,共培养时间为3d,Kan筛选压为100mg/L,头孢霉素所用浓度500mg/L,当菌液浓度OD600=0.6浸染45min。并对得到的再生植株进行了PCR检测,初步证实了目的基因转到了小麦基因组中。
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| 关 键 词： | 小麦 农杆菌 遗传转化 |
Study on Genetic Transformation Conditions of Wheat Mediated by Agrobacterium Tumefaciens |
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| HE Jie,WANG Wei,HU Hai-yan,ZHAO Jun-jie,XIAO Xian-zhou.Study on Genetic Transformation Conditions of Wheat Mediated by Agrobacterium Tumefaciens[J].Seed,2010,29(5). |
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| Authors: | HE Jie WANG Wei HU Hai-yan ZHAO Jun-jie XIAO Xian-zhou |
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| Abstract: | Callus induction regeneration system of wheat,genetic transformation of wheat using and agrobacterium-mediated approach were applied in the research to study the genetic transformation conditions of wheat mediated by agrobacterium tumefaciens.The factors affecting the transformation,such as pre-cultured,co-cultivation,the selective pressure of antibiotic,Cef on the regeneration,agrobacterium concentration and infection periodwere examined.The optimized procedure was as following:after pre-cultured for 1 to ... |
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| Keywords: | wheat agrobacterium tumefaciens genetic transformation |
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