| 红壤区油茶根际解磷细菌的筛选、鉴定及其解磷能力 |
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| 引用本文： | 王舒,张林平,张扬,郝菲菲,肖金香,胡冬南.红壤区油茶根际解磷细菌的筛选、鉴定及其解磷能力[J].林业科学研究,2015,28(3):409-416. |
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| 作者姓名： | 王舒 张林平 张扬 郝菲菲 肖金香 胡冬南 |
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| 作者单位： | 江西农业大学园林与艺术学院,江西南昌,330045 |
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| 基金项目： | 国家自然科学基金项目(31260194)、江西农业大学博士启动经费(09004155) |
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| 摘 要： | 研究油茶根际解无机磷细菌的解磷能力对提高红壤丘陵区土壤磷素利用效率具有重要的指导意义.本研究从油茶根际分离筛选出97株具有解无机磷能力的菌株,采用NBRI-BPB培养基进行复筛获得5株解磷能力较强的解无机磷细菌,并测定其在NBRIP培养基中有效磷含量,采用形态特征、生理生化、Biolog系统和16SrDNA序列分析鉴定细菌种类,确定WB38为耳假单胞菌(Pseudomonas auricularis),WB39(WB75)为杓兰果胶杆菌(Pectobacterium cpripedii),WB53(WB68)为路德维希肠杆菌(Enterobacter ludwigii).研究了解磷菌株WB38在不同条件对解磷能力的影响,结果表明:不同因子对该菌株解磷能力均有显著影响,其中温度对其解磷能力影响最大;该菌株培养的最适碳源和氮源分别为葡萄糖和NH4NO3;解磷细菌WB38解磷的最佳培养条件为A2B1C1D1,即温度28℃,初始pH值6.5,接种量1％,溶氧量25 mL.
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| 关 键 词： | 油茶 解磷细菌 解磷能力 16SrDNA Biolog系统 |
| 收稿时间： | 9/1/2014 12:00:00 AM |
Screening, Identification and Phosphate Solubilizing Capability of Phosphate Solubilizing Bacteria in Rhizosphere of Camellia oleifera Abel at Red Soil Region |
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| WANG Shu,ZHANG Lin-ping,ZHANG Yang,HAO Fei-fei,XIAO Jin-xiang and HU Dong-nan.Screening, Identification and Phosphate Solubilizing Capability of Phosphate Solubilizing Bacteria in Rhizosphere of Camellia oleifera Abel at Red Soil Region[J].Forest Research,2015,28(3):409-416. |
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| Authors: | WANG Shu ZHANG Lin-ping ZHANG Yang HAO Fei-fei XIAO Jin-xiang and HU Dong-nan |
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| Institution: | College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China;College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China;College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China;College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China;College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China;College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China |
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| Abstract: | |
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| Keywords: | Camellia oleifera phosphate-solubilizing bacteria phosphate-solubilizing capability 16S rDNA Biolog system |
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