| 华支睾吸虫吉林分离株的鉴定 |
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| 引用本文： | 刘国兴,陈根源,于建利,吴秀萍,王子见,贺亚奇,刘明远,王峰.华支睾吸虫吉林分离株的鉴定[J].动物医学进展,2011,32(3):37-40. |
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| 作者姓名： | 刘国兴 陈根源 于建利 吴秀萍 王子见 贺亚奇 刘明远 王峰 |
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| 作者单位： | 人兽共患病研究教育部重点实验室,吉林大学人兽共患病研究所,吉林大学畜牧兽医学院,吉林长春,130062 |
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| 基金项目： | 国家自然科学基金项目，国家科技重大专项项目 |
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| 摘 要： | 为了研究吉林省内华支幸吸虫自然分离株的种类及基因变异情况,将镜检含有疑似华支睾吸虫囊蚴的麦穗鱼,经胃蛋白酶消化后获得该寄生虫囊蚴,用比重沉降法去除杂质,同时利用形态学和PCR方法进行种类鉴定.镜检结果表明分离株具有典型的华支睾吸虫囊蚴特征,并且经过PcR方法扩增出了华支睾吸虫的种特异性基因片段,碱基序列与GenBank...
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| 关 键 词： | 华支睾吸虫 囊蚴 分离 鉴定 |
Identification of Clonorchis sinensis Jilin Strain |
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| LIU Guo-xing,CHEN Gen-yuan,YU Jian-li,WU Xiu-ping,WANG Zi-jian,HE Ya-qi,LIU Ming-yuan,WANG Feng.Identification of Clonorchis sinensis Jilin Strain[J].Progress In Veterinary Medicine,2011,32(3):37-40. |
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| Authors: | LIU Guo-xing CHEN Gen-yuan YU Jian-li WU Xiu-ping WANG Zi-jian HE Ya-qi LIU Ming-yuan WANG Feng |
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| Institution: | LIU Guo-xing,CHEN Gen-yuan,YU Jian-li,WU Xiu-ping,WANG Zi-jian,HE Ya-qi,LIU Ming-yuan,WANG Feng(Key Laboratory of Zoonoses,Ministry of Education,Institute of Zoonoses,Jilin University,College of Animal Science and Veterinary Medicine,Changchun,Jilin,130062,China) |
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| Abstract: | In order to research the isolated species and genetic variation of Clonorchis sinensis in Jilin province,the suspected Clonorchis sinensis metacercariae were checked by using microscope from Pseudorasbora parva after removing the dopant by gravity settling process.At the same time,the species was identified by PCR technology and morphology.Microscopic examination showed that they have the typical features of Clonorchis sinensis metacercaria,and the specific gene sequences amplified using PCR methods have 10... |
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| Keywords: | Clonorchis sinensis metacercaria isolation identification |
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