| 96孔板高通量筛选多杀菌素高产菌株的研究 |
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| 引用本文： | 罗莉斯,李能威,李丽,郭伟群,郁建平,张晓琳.96孔板高通量筛选多杀菌素高产菌株的研究[J].中国农业科技导报,2010,12(2):133-137. |
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| 作者姓名： | 罗莉斯 李能威 李丽 郭伟群 郁建平 张晓琳 |
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| 作者单位： | (1.贵州大学生命科学学院, 贵阳 550025,2.国家粮食局科学研究院, 北京 100037) |
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| 基金项目： | “十一五”国家科技支撑计划项目(2006BAD08B02-1)资助 |
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| 摘 要： | 考察了刺糖多孢菌在96孔板固体培养基和液体培养基中的生长情况，建立了96孔板高通量筛选多杀菌素高产菌株的方法。结果表明：96孔板三明治盖能同时满足过滤杂菌和刺糖多孢菌生长耗氧所需，刺糖多孢菌在96孔板固体培养基中的生长情况与传统斜面培养相当，种子培养最适种龄为60 h，每孔发酵培养基装液量为0.4 mL。与传统摇瓶发酵筛选平台相比，该方法能显著提高多杀菌素高产菌株的筛选效率。
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| 关 键 词： | 刺糖多孢菌 多杀菌素 96孔板 高通量筛选 |
| 收稿时间： | 2010-01-13 |
| 修稿时间： | 2010-03-18 |
Studies on High Throughput Screening for High Spinosad-producing Strains with 96-well Plates |
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| LUO Li-si,LI Neng-wei,LI Li,GUO Wei-qun,YU Jian-ping,ZHANG Xiao-lin.Studies on High Throughput Screening for High Spinosad-producing Strains with 96-well Plates[J].Journal of Agricultural Science and Technology,2010,12(2):133-137. |
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| Authors: | LUO Li-si LI Neng-wei LI Li GUO Wei-qun YU Jian-ping ZHANG Xiao-lin |
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| Institution: | (1.College of Life Sciences, Guizhou University, Guiyang 550025|2.Academy of State Administration of Grain, Beijing 100037, China) |
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| Abstract: | Based on the growth of Saccharopolyspora spinosa in both solid and liquid medium,a high throughput method was established to screen high spinosad-producing strains with 96-well plates.The results showed that applying the sandwich covers for 96-well plates could meet the requirement of bacteria filtration and oxygen supply for the growth of S.spinosa.S.spinosa grew well in both 96-well plate and slant culture.The optimum seed growing time was 60 h;the broth content of each well was 0.4 mL.Comparing with the ... |
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| Keywords: | Saccharopolyspora spinosa spinosad 96-well plate high throughput screening |
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