冬枣花药愈伤组织的诱导与增殖

目的]获得冬枣花药胚性愈伤组织。方法]以冬枣花药为试材，研究了碳源种类、基本培养基、植物生长调节剂对冬枣花药愈伤组织诱导及增殖的影响。结果]麦芽糖为诱导冬枣花药愈伤组织的最适碳源，MS为最适基本培养基，1．0mg／LTDZ为最适诱导与增殖生长调节剂浓度。结论]在MS＋麦芽糖20g／L＋TDZ1．0mg／L的培养基中，冬枣花药愈伤组织诱导率最高，达86．8％。获得的愈伤组织可进行增殖，愈伤组织呈黄绿色颗粒状，为胚性愈伤。

Anther Callus Induction and Propagation of Ziziphus jujuba 'Dongzao'

Objective] The study aimed to obtain callus from Dongzao (Ziziphus jujuba Mill.).Method] With anthers of Z.jujuba as test materials, the effects of carbon source kinds, basic medium, and plant growth regulator on the callus induction and propagation of Z.jujuba anthers were studied.Result]The results indicated that maltose was the optimum carbon source, MS was the optimum basic medium, and 1.0 mg/L TDZ was the optimum concentration of growth regulator for callus induction and propagation.In the medium of MS+20 g/L maltose + 1.0 mg/L TDZ, the callus induction rate was highest(86.8%).The induced callus was proliferated on MS medium supplemented with maltose 20 g/L+TDZ 1.0 mg/L.And the callus was embryogenic callus with kelly and grain-shape.