Aspergillus flavus-secreted proteins during an in vivo fungal-cotton carpel tissue interaction |
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| Institution: | 1. Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, Harbin Normal University, Harbin 150025, China;2. Key Laboratory of Synthesis of Functional Materials and Green Catalysis, Colleges of Heilongjiang Province, Harbin Normal University, Harbin 150025, China;3. Department of Biochemical Engineering, Harbin Institute of Technology, Harbin 150090, China |
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| Abstract: | Cotton bolls (Gossypium hirsutum) were inoculated with Aspergillus flavus to investigate extracellular hydrolases produced during infection. Fungal proteins from the infection site were isolated and analyzed by SDS-PAGE. Protein bands were excised and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified several proteins including an exoglucanase 1 precursor (AFL2G_03805), a hypothetical protein similar to rhamnogalacturonan lyase A (AFL2G_05136), and a hypothetical protein similar to pectate lyase A (AFL2G_05954). We also matched three peptides to an oryzin precursor protein (AFL2G_01995). Our findings support the conclusion that glucanase, pectinolytic, and proteolytic proteins are important for fungal maceration of cotton carpel tissue during infection. |
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| Keywords: | Glucanase Pectinolytic Proteinase Virulence factor Carpel tissue |
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