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| 沼泽红假单胞菌产类胡萝卜素发酵条件优化 | |
| 引用本文： | 崔艺久,王悦,牛明芬,黄剑,韩梅. 沼泽红假单胞菌产类胡萝卜素发酵条件优化[J]. 沈阳农业大学学报, 2012, 43(2): 225-228 |
| 作者姓名： | 崔艺久 王悦 牛明芬 黄剑 韩梅 |
| 作者单位： | 沈阳农业大学土地与环境学院,沈阳,110161 |
| 基金项目： | 国家水体污染控制与治理科技重大专项项目 |
| 摘 要： | 利用单因素试验与正交试验的方法,对沼泽红假单胞菌B9(Rhodopsedomonas palustris B9)产类胡萝卜素培养基和培养条件进行优化。结果表明:最佳培养基成分为:KH2PO40.6g,K2HPO40.9g,酵母膏1.5 g,蛋白胨2.3g,MgSO40.2g,CaCl20.3g,NaCl0.5g,最佳培养条件为:接种量为8%,温度为26℃,pH值为自然pH值。培养7d后,类胡萝卜素产量可达2.863mg·g-1(以干重计)。 |
| 关 键 词： | 沼泽红假单胞菌 类胡萝卜素 生长条件 优化 |
Rhodopseudomonas palustris Optimization of Fermentation Conditions for Production of Carotenoids |
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| CUI Yi-jiu , WANG Yue , NIU Ming-fen , HUANG Jian , HAN Mei. Rhodopseudomonas palustris Optimization of Fermentation Conditions for Production of Carotenoids[J]. Journal of Shenyang Aricultural University, 2012, 43(2): 225-228 | |
| Authors: | CUI Yi-jiu WANG Yue NIU Ming-fen HUANG Jian HAN Mei |
| Affiliation: | (College of Land and Environment,Shenyang Agricultural University,Shenyang 110161,China) |
| Abstract: | Using the methods of single factor and orthogonal experimental,culture medium and culture condition for Rhodopsedomonas palustris B9 to produce carotenoids were investigated.As results,the best culture medium are yeast extract 1.5g,peptone 2.3g,KH2PO4 0.6g,K2HPO4 0.9g,NaCl 0.5g,water 1000mL in its natural pH value;and the best culture conditions are 8% inoculating concentration,26℃.After 7 days cultivation,the total carotenoids reached up to 2.863 mg·g-1(dry weight calculation). |
| Keywords: | Rhodopseudomonas palustris carotenoids growth condition optimization |
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