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| 人IL-1Ra基因的克隆及原核表达的研究 | |
| 引用本文： | 双宝,李明,李慧,矫洪涛,王晴,刘忠华,向文胜. 人IL-1Ra基因的克隆及原核表达的研究[J]. 东北农业大学学报, 2011, 42(3) |
| 作者姓名： | 双宝 李明 李慧 矫洪涛 王晴 刘忠华 向文胜 |
| 作者单位： | 1. 东北农业大学生命科学学院,哈尔滨,150030 2. 东北农业大学司法鉴定中心,哈尔滨,150030 |
| 基金项目： | 国家自然科学基金面上项目 |
| 摘 要： | IL-1Ra是第一个被发现的天然存在的细胞因子拮抗剂,结合于IL-1受体后不引起IL-1效应。文章通过RT-PCR从人的胎盘组织中克隆了人IL-1Ra基因,使其在大肠杆菌DH5α中重组表达。结果表明,克隆到了460bp左右的目的基因,并在大肠杆菌中成功表达了18ku左右的目的蛋白,目的蛋白在大肠杆菌中表达量占总蛋白20%左右,为IL-1Ra的进一步研究及应用奠定了基础。 |
| 关 键 词： | 人IL-1Ra 基因重组 原核表达 |
Study on clone and prokaryotic expression of human IL-1Ra |
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| SHUANG Bao,LI Ming,LI Hui,JIAO Hongtao,WANG Qing,LIU Zhonghua,XIANG Wensheng. Study on clone and prokaryotic expression of human IL-1Ra[J]. Journal of Northeast Agricultural University, 2011, 42(3) | |
| Authors: | SHUANG Bao LI Ming LI Hui JIAO Hongtao WANG Qing LIU Zhonghua XIANG Wensheng |
| Abstract: | IL-1Ra is the natural antagon of cellular factor which was found firstly.And its binds receptor of IL1-Ra but not causing any reactions of IL-1.In this study,cloning of IL-1Ra gene from placenta tissue of human by RT-PCR and recombinant expression of this gene in E.coli DH5α which is prokaryotic organism were completed.As a result,a DNA which was about 450 bp was cloned and IL-1Ra which was about 18 ku was expressed.IL-1Ra expressed in E.coli(DE3) was about 20% of the total proteins.It makes possible to study and utilize IL1-Ra further. |
| Keywords: | human IL1-Ra gene recombination prokaryotic expression |
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