| 尿激酶原基因在小鼠体内的表达 |
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| 引用本文： | 邓雯,李银聚,董淑丽,庞有志,伦柏楠,王天奇,王建平,康怀彬.尿激酶原基因在小鼠体内的表达[J].河南农业大学学报,1999,33(4):383-385. |
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| 作者姓名： | 邓雯 李银聚 董淑丽 庞有志 伦柏楠 王天奇 王建平 康怀彬 |
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| 作者单位： | 洛阳农业高等专科学校,河南 洛阳 471003 |
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| 基金项目： | 河南省科委自然科学基金!（964012800） |
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| 摘 要： | 将构建的２种重组表达质粒ｐＳＶ２－ｕＰＡ和ｐｃＤＮＡ３－ｕｐＡ分别直接注入小鼠骨骼肌，观察了Ｓｃｕ－ＰＡ ｃＤＮＡ在小鼠体内的表达情况。体外凝血试验显示注射质粒ＤＮＡ后，第５ｄ凝血时间有所延长，第１５ｄ凝血时间延长幅度最大，较对照组平均延长６ｍｉｎ左右；第２０ｄ体内诱导产生ＤＩＣ，体外凝血时间试验组较对照组延长一倍，抗凝效果明显，２种表达质粒在体内的表达水平无明显差异。
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| 关 键 词： | 小鼠 尿激酶原 肌肉注射 凝血 |
Expression of Scu-PA cDNA gene in mouse |
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| DENG Wen,LI Yin-ju,DONG Shu-li,et
al.Expression of Scu-PA cDNA gene in mouse[J].Journal of Henan Agricultural University,1999,33(4):383-385. |
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| Authors: | DENG Wen LI Yin-ju DONG Shu-li et
al |
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| Institution: | DENG Wen,LI Yin-ju,DONG Shu-li,et
al
(Luoyang Agricultural College,Luoyang 471003,China) |
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| Abstract: | Two expression plasmids,pSV 2 uPA and pcDNA 3 uPA,containing the single chain Urokinase type plasmoneign activator (Scu PA) cDNA gene were constructed.The Scu PA expressed in mouse muscle was observed by direct injection of pSV 2 uPA and pcDNA 3 uPA into the muscle.The result showed that the prolongation of blood clotting time could be discerned at 5th day after the injection of the recombinant plasmids,and the peak occurred on the 15th day.On an average,the blood coagulation time prolonged for 6 min compared with the CK.Disseminated intravascular Coagulation (DIC) was induced on the 20th day.Compered with normal mouse,blood clotting time doubled,and there was no obvious difference between the expression levels of the two plasmids. |
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| Keywords: | mouse Scu PA muscle injection blood clotting |
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