| 响应面法优化青蛤降血压肽酶解制备工艺 |
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| 引用本文： | 詹秋桂,李杰煌,施鹏飞,雷鸣,罗李王,余方苗.响应面法优化青蛤降血压肽酶解制备工艺[J].安徽农业科学,2018,46(5):7-9,24. |
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| 作者姓名： | 詹秋桂 李杰煌 施鹏飞 雷鸣 罗李王 余方苗 |
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| 作者单位： | 浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022;浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022;浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022;浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022;浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022;浙江海洋大学食品与医药学院,浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022 |
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| 基金项目： | 浙江省大学生科技创新项目，浙江省教育厅项目 |
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| 摘 要： | 目的]采用响应面法优化青蛤降血压肽的酶解制备工艺。方法]以青蛤肉为原料,木瓜蛋白酶为酶种,血管紧张素转换酶抑制率为指标,在单因素试验的基础上,选择pH、酶解时间和料液比为影响因素,进行3因素3水平的Box-Behnken试验设计,采用响应面法分析3个因素对青蛤降血压肽制备工艺的影响。结果]木瓜蛋白酶酶解制备青蛤降血压肽的最佳工艺条件为pH 5.8、酶解时间8.4 h、料液比1∶3.1,该条件下酶解产物对血管紧张素转换酶抑制率达93.58%。结论]该研究为青蛤的高值化利用和降血压肽的制备提供了新思路。
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| 关 键 词： | 青蛤 木瓜蛋白酶 降血压肽 响应面法 |
Optimization of Enzymatic Hydrolysis Process for Preparation of Antihypertensive Peptides from Cyclina sinensis by Response Surface Methodology |
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| Abstract: | Objective] Enzymatic hydrolysis process for preparing antihypertensive peptides from Cyclina sinensis was optimized by using response surface methodology.Method] Taking angiotensin-converting enzyme (ACE) inhibition rate as index,a 3-factor and 3-level Box-Behnken design coupled with response surface analysis was carried out to investigate the effects of pH,hydrolysis time and solid-liquid ratio on ACE inhibition rate.Result] The optimal conditions for preparing antihypertensive peptides from Cyclina sinensis by using papain were as followed:pH 5.8,hydrolysis time 8.4 h and solid-liquid ratio1:3.1,and the ACE inhibition rate of enzymatic hydrolysates were up to 93.58％ under these conditions.Conclusion] This study provides novel ideas for the high-value use of Cyclina sinensis and preparing antihypertensive peptides. |
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