外源一氧化氮对七叶一枝花种子萌发的影响

目的]探究外源一氧化氮(NO)处理对七叶一枝花种子萌发及其相关生理指标的影响。方法]检测了不同浓度硝普钠(NO供体)对七叶一枝花种子萌发的促进效果,并在此基础上,探讨了最适浓度硝普钠处理后七叶一枝花种子萌发过程中淀粉、可溶性蛋白以及可溶性糖等含量的变化情况。结果]100μmol/L硝普钠促进七叶一枝花种子的萌发效果最为明显;与对照相比,不同时间段下硝普纳处理组种子中,NO含量在45~90 d显著高于对照组;可溶性糖含量总体上高于对照,且在15~45 d有显著差异;淀粉含量在15~45 d显著低于对照组;β淀粉酶活力在萌发早期显著高于对照组,且总淀粉酶活力的变化与β淀粉酶活力变化趋势一致;可溶性蛋白含量分析中,处理组与对照组无显著差异,都有随萌发推进而呈现下降的趋势。结论]100μmol/L硝普钠促进七叶一枝花种子的萌发可能是通过提高种子萌发早期淀粉酶的活性,促进淀粉的水解和可溶性糖的产生,以及提高萌发期间种子内源NO的含量而实现。

Effects of Exogenous Nitric Oxide on the Seed Germination of Paris polyphylla var.chinesis

Objective] The research aimed to study the effects of exogenous nitric oxide(NO) on the germination and the related physiological indices of seeds in Paris polyphylla.Method] The effects of different concentration of sodium nitroprusside (NO donor) on seed germination in Paris polyphylla was detected.And on the basis of this,the changes of starch,soluble protein and soluble sugar in the process of seed germination of Paris polyphylla were tested by the optimal concentration of sodium nitroprusside.Result]The effect of 100 μ mol/L sodium nitroprusside on seed germination was the most obvious.Comparison with the control,in the nitroprusside group under different time periods,NO content in 45-90 d was significantly higher than the control group;The soluble sugars content were higher than that in the control,and there was a significant difference between 15 and 45 days.Starch content was significantly lower than that in the control group in 15-45 days.The activity of β-amylase in the early stage of germination was significandy higher than that in the control group,and the change of total amylase activity was in accordance with the change trend of amylase activity.Soluble protein content analysis,the treatment group and the control group had no significant difference,on the whole showed a downward trend with the germination and progress.Conclusion] 100 μmol/L sodium nitroprusside promoting germination effect of Paris polyphylla may be achieved by increasing the activity of amylase in the early stage of seed germination,promoting the hydrolysis of starch and producing the soluble sugar,and improving the contents of endogenous NO during germination.