| 百脉根试管苗对NaCl的敏感性检测 |
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| 引用本文： | 谭化,高明,于淑梅,韦正乙,李海云,刘艳芝,张东良. 百脉根试管苗对NaCl的敏感性检测[J]. 吉林农业科学, 2010, 35(2): 14-16 |
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| 作者姓名： | 谭化 高明 于淑梅 韦正乙 李海云 刘艳芝 张东良 |
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| 作者单位： | 吉林省农业科学院生物技术中心,长春,130033;吉林省抚松县农业局,吉林,抚松,134500 |
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| 基金项目： | 抗逆聚合表达载体的构建及在模式中功能验证(20060548)项目资助 |
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| 摘 要： | 各种植物对NaCl都具有一定的敏感性。本实验利用野生型百脉根组织培养再生苗为研究材料,在附加不同浓度NaCl的MS培养基上观察其生长及生根状态,确定百脉根试管苗对NaCl的临界耐受能力,目的是为筛选转耐盐基因百脉根抗盐性植株提供实验依据,同时也可以初步检测所克隆基因的耐盐效果。实验结果:NaCl浓度为0.7%以上的处理,试管苗叶片发白,长势弱,不生根或根停止伸长变褐;NaCl浓度为0.7%(不包括0.7%)以下的处理中,试管苗叶片绿色,长势旺盛,根系较多,与对照(NaCl浓度为0)试管苗状态接近。
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| 关 键 词： | 百脉根 NaCl浓度 生长及生根 |
Detection of NaCl Tolerance of Lotus corniculatus L.Regenerants |
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| TAN Hua,GAO Ming,YU Shu-mei,WEI Zheng-yi,LI Hai-yun,LIU Yan-zhi,ZHANG Dong-liang. Detection of NaCl Tolerance of Lotus corniculatus L.Regenerants[J]. Journal of Jilin Agricultural Sciences, 2010, 35(2): 14-16 |
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| Authors: | TAN Hua GAO Ming YU Shu-mei WEI Zheng-yi LI Hai-yun LIU Yan-zhi ZHANG Dong-liang |
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| Affiliation: | 1.Biotechnology Research Center;Academy of Agricultural Sciences of Jilin Province;Changchun 130033;2.Bureau of Agricultural of Fusong County Jilin Province;Jilin Fusong 134500;China |
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| Abstract: | Most plants are sensitive to NaCl.In order to identify the NaCl tolerance of Lotus corniculatus L.regenerants in vitro,the growth and rooting of these regenerants cultured in the MS medium supplemented with different concentration NaCl were determined in the experiment.The results showed that plants grew slowly and could not rooting,their leaves turned white when NaCl concentration was above 0.7%.When NaCl concentration was below 0.7%,the plants grew as same as the control.This method could be used in ident... |
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| Keywords: | Lotus corniculatus L. NaCl concentration Growth and rooting |
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