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| 合作猪Nramp1基因第六内含子多态性分析 | |
| 引用本文： | 李娟娟,马小军,王立贤,张小丽,李小飞. 合作猪Nramp1基因第六内含子多态性分析[J]. 甘肃农业大学学报, 2010, 45(2) |
| 作者姓名： | 李娟娟 马小军 王立贤 张小丽 李小飞 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;甘肃省草食动物生物技术重点实验室,甘肃,兰州,730070 2. 中国农业科学院北京畜牧兽医研究所,北京,100094 3. 甘肃农业大学动物医学院,甘肃,兰州,730070 |
| 基金项目： | 甘肃省自然科学基金(096RJZA005) |
| 摘 要： | 采用PCR-SSCP方法检测了135头合作猪Nramp1基因第六内含子的多态性.结果表明:序列比对分析发现10个新的核苷酸多态位点,其中,转换7个,颠换2个,插入1个;试验群体发现5个基因型,即AA、AB、BB、CC、AC,其中B等位基因为优势等位基因,AB基因型为优势基因型;经χ2适合性检验,该群体处于Hardy-Wein-berg平衡状态(P0.05);Nramp1基因遗传多态指数中,杂合度为0.617 4,多态信息含量为0.537 3,有效等位基因数为2.613 7. |
| 关 键 词： | 合作猪 Nramp1基因 PCR-SSCP 多态性 |
Polymorphism of the Nramp1 gene intron 6 in Hezuo pig |
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| LI Juan-juan,MA Xiao-jun,WANG Li-xian,ZHANG Xiao-li,LI Xiao-fei. Polymorphism of the Nramp1 gene intron 6 in Hezuo pig[J]. Journal of Gansu Agricultural University, 2010, 45(2) | |
| Authors: | LI Juan-juan MA Xiao-jun WANG Li-xian ZHANG Xiao-li LI Xiao-fei |
| Affiliation: | 1.College of Veterinary Medicine;Gansu Agricultural University;Lanzhou 730070;China;2.Gansu Provience Key Laboratory of Grazing Animal Biotechnology;3.Insitute of Animal Science and Veterinary Medicine;Chinese Academy of Agricultural Sciences;Beijing 100094;China |
| Abstract: | In order to explore the molecular mechanism of disease resistance of Hezuo pigs,the PCR-SSCP method was used to analyse the Nramp1 gene intron 6 polymorphisms in 135 Hezuo pigs.Sequencing analysis found ten new nucleotide polymorphisms in intron 6,which mainly came from point mutation,including transition of 7,transversion of 2 and insertion of 1.Five genotypes AA,AB,BB,AC and CC were detected,allele B and genotype AB were predominant.Chi-square test indicated that the polymorphic locus in Hezuo pigs were a... |
| Keywords: | Hezuo pig Nramp1 gene PCR-SSCP polymorphism |
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