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| DEAE-Sepharose Fast Flow分离纯化刺芹侧耳木质素降解酶 | |
| 引用本文： | 贾俊睿,陈敏,梁新乐,李锋. DEAE-Sepharose Fast Flow分离纯化刺芹侧耳木质素降解酶[J]. 安徽农业科学, 2009, 0(2): 448-450 |
| 作者姓名： | 贾俊睿 陈敏 梁新乐 李锋 |
| 作者单位： | 浙江工商大学食品与生物工程学院,浙江杭州,310012;浙江工商大学食品与生物工程学院,浙江杭州,310012;浙江工商大学食品与生物工程学院,浙江杭州,310012;浙江工商大学食品与生物工程学院,浙江杭州,310012 |
| 基金项目： | 浙江省科技厅新苗计划(2007R40G2050021); 浙江省自然科学基金资助(Y505334) |
| 摘 要： | [目的]为Pleurotus eryngii—Co60-7木质素降解酶的分离纯化和综合利用提供试验依据。[方法]采用DEAE—Sepharose^TM Fast Flow离子交换介质，分别考察缓冲液pH值、流速和洗脱方式等对刺芹侧耳木质素降解酶分离纯化的影响，确定了最佳分离纯化层析条件。[结果]DEAE-Sephalose^TM Fast Flow分离纯化Pleurotus eryngii-Co60-7木质素降解酶的最佳层析条件为：选择20mmol／L，pH值为5．0醋酸钠一醋酸缓冲体系，3ml／min的流速，进行分步洗脱（100、200～300和1000mmoL／L NaCl的三步洗脱），可较好地实现刺芹侧耳发酵液木质素降解酶初分，该纯化操作目标蛋白回收率达85％，纯化分离因素为2．71。[结论]该技术在分离纯化刺芹侧耳木质素降解酶上可行，具有潜在的工业应用价值。 |
| 关 键 词： | Pleurotus eryngii-Co60-7 木质素降解酶 离子交换层析 DEAE-SepharoseTM Fast Flow |
Purification of Ligninolytic Enzymes from Pleurotus eryngii by DEAE-Sepharose Fast Flow |
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| JIA Jun-rui et al. Purification of Ligninolytic Enzymes from Pleurotus eryngii by DEAE-Sepharose Fast Flow[J]. Journal of Anhui Agricultural Sciences, 2009, 0(2): 448-450 | |
| Authors: | JIA Jun-rui et al |
| Affiliation: | JIA Jun-rui et al(College of Food Science & Biotechnology Engineering,Zhejiang Industry , Business University,Hangzhou,Zhejiang 310012) |
| Abstract: | [Objective] The aim of the research was to provide the experimental basis for the purification and the comprehensive utilization of the Ligninolytic enzymes from Pleurotus eryngii-Co60-7 strain.[Method] Purification of the Ligninolytic enzymes from Pleurotus eryngii-Co60-7 strain was investigated by the anion-exchange media DEAE-SepharoseTM Fast Flow,by optimizing pH value of the buffer solution,flow rate and elution condition,and its optimal condition of the chromatography was confirmed.[Results] The optim... |
| Keywords: | Pleurotus eryngii-Co60-7 Ligninolytic enzymes Ion exchange chromatography DEAE-SepharoseTM Fast Flow |
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