甘蓝型油菜柱头特异启动子的克隆和序列分析

芸薹属自交不亲和相关基因SLR1只在柱头中特异表达，根据已知的SLR1基因启动子序列设计特异引物，用PCR法从几个甘蓝型油菜品种和品系中分离得到相应的启动子片断，进行序列比较分析后，并构建它们与GFP融合的植物表达载体，转化拟南芥，验证所分离到的启动子的时空特异性。研究表明，来自自交不亲和羽衣甘蓝和来自自交亲和的甘蓝型油菜的SLR1启动子具有相似的序列结构和相同的组织特异表达功能。该结果将为下一步研究S-locus基因在甘蓝型油菜中的表达和相互作用奠定基础。

Isolation and Sequence Analysis of the Stigma-specific Promoter from Brassica napus

SLR1 gene is a stigma-specific S-locus related gene widespread in Brassica. Primers derived from the SLR1 promoter sequence of B. napus var. westar were used to perform PCR analysis of several B. napus varieties, so as to isolate corresponding promoters of self-compatible B. napus. The fragments achieved by PCR were cloned and sequenced, and then fused to the GFP gene, replaced the 35S promoter in the plant expressed vector pFGC-eGFP. The vector was transformed to Arabidopsis by Agrobacterium-mediated transformation. By sequences analysis and fluorescence detection, the results showed that the SLR1 promoter in self-compatible B. napus had similar structure and function with that in self-incompatible B. oleracea which had been reported previously.