不同浓度吐温-80与白僵菌分生孢子的相容性测定 |
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引用本文: | 任巧云,殷宏,关贵全,马米玲,刘爱红,李有全,刘军龙,牛庆丽,金玉荣,王子坚,罗建勋. 不同浓度吐温-80与白僵菌分生孢子的相容性测定[J]. 安徽农业科学, 2009, 37(18): 8555-8555 |
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作者姓名: | 任巧云 殷宏 关贵全 马米玲 刘爱红 李有全 刘军龙 牛庆丽 金玉荣 王子坚 罗建勋 |
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作者单位: | 中国农业科学院兰州兽医研究所家畜疫病病菌生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州,730046 |
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基金项目: | 国家支撑计划,国家高技术研究发展计划(863计划) |
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摘 要: | [目的]筛选吐温-80的最佳试验浓度。[方法]采用营养液萌发法测定11种不同浓度的吐温-80与白僵菌分生孢子的生物相容性。[结果]结果表明,吐温-80在浓度为0.0075%、0.0250%、0.0500%和0.1000%时白僵菌的孢子萌发率均达到95%以上,且差异不大,对白僵菌的生长影响较小。[结论]当吐温-80浓度小于0.1000%(含0.1000%)时可考虑应用于白僵菌的相容性试验中。
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关 键 词: | 白僵菌 吐温-80 |
Determination of Biological Compatibility between Tween 80 and Beauveria bassiana Conidia |
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Affiliation: | REN Qiao-yun et al ( State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046) |
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Abstract: | [Objective] The research aimed to screen out the best concentration of Tween 80.[Method]The biological compatibility of B.bassiana conidia and 11 kinds of Tween 80 was determined by germination conidial in nutritional liquid.[Result] The results showed that germination rates of Beauveria bassiana exceed 95% when the concentration of Tween 80 was 0.007 5%、0.025 0%、0.050 0% and 0.100 0%.[Conclusion]Tween 80 can be use in the compatibility experiment of B.bassiana if the concentration of Tween 80 is lower than 0.100 0%(including 0.100 0%). |
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Keywords: | Beauveria bassiana Tween 80 |
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