Characterization of ileal bacterial microbiota in newly-weaned pigs in response to feeding lincomycin, organic acids or herbal extract |
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| Authors: | Joshua Gong Hai Yu Ting Liu Mei Li Weiduo Si Cornelis F.M. de Lange Cate Dewey |
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| Affiliation: | aGuelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Ontario, Canada N1G 5C9;bDepartment of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1;cDepartment of Population Medicine, University of Guelph, Guelph, Ontario, Canada N1G 2W1 |
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| Abstract: | The changes of ileal bacterial microbiota in pigs during the first 2 weeks post-weaning in response to feeding lincomycin, organic acids or herbal extract were characterized using 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis (DGGE) profiling, DNA sequencing, and real-time PCR (QPCR) techniques. Both time post-weaning and the dietary treatments resulted in a shift of the microbiota composition. While time post-weaning mostly influenced Clostridium group, the feed additives increased the population of Lactobacillus and related lactic acid bacteria by ≥ 3-fold. |
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| Keywords: | Pigs Ileal microbiota Lincomycin Organic acids Herbal extract 16S rRNA |
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