不同浓度腐殖酸对油菜苗期生长及生理特性的影响

以油菜品种Cao221167为试验材料,探究水培条件下腐殖酸对作物苗期生长及生理特性的影响。通过设置不同浓度的腐殖酸(0；1‰；2‰；3‰；4‰；5‰)研究其对油菜幼苗生物量、株高、根系特征、叶绿素含量、MDA含量以及抗氧化酶活性等的影响。结果]结果表明,1‰浓度腐殖酸时显著影响油菜幼苗的生物量(地上部干鲜重分别比对照增加18%和12.20%,地下部干鲜重分别比对照增加41.67%和54.70%),添加腐殖酸可以明显提高油菜幼苗根系活力；浓度为1‰时显著促进根系的生长(根长、总根长和根表面积分别比对照增加20.36%、70.49%、30.43%)。在1‰～5‰浓度范围内油菜细胞内抗氧化酶活性先减小后增大,叶绿素含量先增大后减小。因此,一定浓度的腐殖酸可以促进油菜的生长,且1‰～2‰浓度的腐殖酸是油菜苗期生长的适宜浓度,浓度为3‰～5‰时对油菜幼苗的生长具有胁迫作用。

Effects of Different Concentrations of Humic Acid on Growth and Physiological Characteristics of Rape Seedlings

Hydroponic experiment were done to investigate the effects of humic acid on growth and physiological characteristics of crops at seedling stage, and the tested material is Cao221167.Method]The experiment set up different concentrations of humic acid(0；1‰；2‰；3‰；4‰；5‰) to study the effects on the biomass, plant height, root characteristics, chlorophyll content, MDA content and antioxidant enzyme activity of rapeseed seedlings.Result] The results showed: when the humic acid concentration was 1‰,the biomass of rape seedling was significantly affected(The dry weight and fresh weight of the shoots increased by 18% and 12.20% respectively, and the dry weight and fresh weight of the underground increased by 41.67% and 54.70% respectively). Added humic acid could significantly improve the root activity of rapeseed seedlings; when the concentration was 1 ‰,it can significantly promoted the root growth(root length, total root length and root surface area increased by 20.36%, 70.49% and 30.43% respectively compared with the control). In the range of 1 ‰ ~ 5 ‰, with the increased of humic acid concentration, the activity of antioxidant enzymes in rape cells decreased first and then increased, and the chlorophyll content increased first and then decreased. Conclusion]So, a certain concentration of humic acid can promote the growth of rape,and 1 ‰ and 2 ‰ concentration of humic acid is the appropriate concentration of rape growth, and when the concentration is 3 ‰ ~ 5 ‰, it inhibits the growth of rape.