| EMS离体诱变和抗大花萱草叶枯病突变体的筛选 |
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| 引用本文： | 杨丽莉,刘化涛,杨睿,常建忠,郭建民,畅平.EMS离体诱变和抗大花萱草叶枯病突变体的筛选[J].中国农学通报,2018,34(4):123-130. |
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| 作者姓名： | 杨丽莉 刘化涛 杨睿 常建忠 郭建民 畅平 |
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| 作者单位： | 山西省农业科学院旱地农业研究中心,山西省农业科学院旱地农业研究中心,山西省农业科学院旱地农业研究中心,山西省农业科学院旱地农业研究中心,山西省农业科学院园艺研究所,西北农林科技大学风景园林艺术学院 |
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| 基金项目： | 山西省重点研发计划“甲基磺酸乙酯(EMS)离体诱导大花萱草抗病突变体的研究”(201603D221017-1)；山西省农业科学院重点项目“EMS 诱变大花萱草抗病突变体的研究”(YZD1513)；山西省农业科学院特色农业攻关项目“EMS 诱变大花萱草突变体的产生及新种质资源创制” (YGG17037)。 |
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| 摘 要： | 利用甲基磺酸乙酯(EMS)诱变大花萱草‘红运’愈伤组织和分化芽,目的是获得抗叶枯病愈伤组织和分化芽突变体。结果表明：0.50%~0.75% EMS处理愈伤组织60 min；0.75%~1.00% EMS处理分化芽60min,分别获得“半致死剂量”效应。对存活的愈伤组织进行毒素浓度递增的多步筛选(10% 30 d—20% 30 d)；分化芽80%毒素21d的一步筛选。经定向筛选,初步获得抗叶枯病分化芽突变体103株,有待进一步进行抗病鉴定。愈伤组织未能获得抗病突变体再生植株。
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| 关 键 词： | 东方百合 东方百合 试管鳞茎 培育方式 |
| 收稿时间： | 2017/9/11 0:00:00 |
| 修稿时间： | 2018/2/1 0:00:00 |
EMS Mutagenesis in Vitro and Screening of Resistant Mutant to Kabatiella microsticta of Hemerocallis middendorfii |
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| Abstract: | The calli and differentiation buds from ovary of Hemerocallis hybrida were mutagenized in vitro by different concentrations of EMS (ethyl methane sulfonate) and screened by toxin extracts after the toxicity testing of Kabatiella microsticta on Hemerocallis hybrida. The results showed that 0.50%-0.75% EMS treated callus 60 min, 0.75%-1.00% EMS treated differentiation bud 60 min, and obtained semi lethal dose effect respectively. The surviving calli were gradually screened for bacterial toxin concentration (10%, 30 days- 20%, 30 days), and the surviving differentiated buds were screened for 21 days with 40% toxin, one step solution. 103 mutants of leaf blight from bud differentiation were obtained by directional screening, and further identification was needed. Calli failed to obtain resistant mutant plants. |
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| Keywords: | induced mutants resistant mutants Ethyl methanesulfonate(EMS) in vitro selection Hemerocallis hybrida Kabatiella microsticta |
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