金银花花器发育时期与有效成分含量的关系

为研究金银花花器发育时期与有效成分含量的关系，以幼蕾期、青蕾期、白蕾期、银花期、金花期5 个不同发育时期的金银花样品为试验材料，采用高效液相色谱法(HPLC)法对绿原酸、咖啡酸、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C的含量、累积量及比例进行研究。结果表明：（1）绿原酸、咖啡酸、木犀草苷以及异绿原酸A含量在青蕾期最高，分别高达27.29、0.22、0.84、0.36 mg/g，而异绿原酸B、异绿原酸C和总异绿原酸含量峰值出现在幼蕾期，分别为7.22、1.77、9.30 mg/g。（2）绿原酸、咖啡酸、异绿原酸A、异绿原酸B、异绿原酸C 和总异绿原酸累积量均在白蕾期最高，分别为82.19、4.12、5.56、101.67、23.05、130.07 μg/朵，仅有木犀草苷累积量最大值出现在青蕾期（10.39 μg/朵）。（3）随着金银花花器发育，绿原酸与木犀草苷比例先降低后增高，而异绿原酸A所占比例逐渐上升，异绿原酸B、C所占比例逐渐降低。金银花有效成分含量、累积量以及比例与发育时期密切相关，最适采收期为白蕾期。

The Relationship Between Floral Organ Development Period and Effective Component Content of Lonicera japonica

To study the relationship between floral organ development period and effective component content of Lonicera japonica, samples at five different developmental stages of L. japonica were collected and analyzed for the content, accumulative amount and ratio of chlorogenic acid, caffeic acid, luteolin, isopropenic acid A, chloro?genic acid B and isochromatic acid C by HPLC method. The results showed that: (1) the contents of chlorogenic acid, caffeic acid, mignonette glycosides and isochlorogenic acid A were the highest in green bud period, respec?tively reached 27.29, 0.22, 0.84, 0.36 mg/g, but the peak content of isochlorogenic acid B, isochlorogenic acid C and total isochlorogenic acid appeared in the infant stage, they were respectively 7.22, 1.77, 9.30 mg/g; (2) the cumulant of chlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and the total isochlorogenic acid were the highest in white bud period, they were respectively 82.19, 4.12, 5.56, 101.67, 23.05, 130.07 μg/bloom; however the maximum cumulant of mignonette glycosides appeared in the green bud stage (10.39 μg/bloom); (3) with the development of floral organ, the ratio of chlorogenic acid and mi? gnonette glycosides decreased first and then increased, the ratio of isochlorogenic acid A increased, while the ra?tio of isochlorogenic acid B and C reduced. In conclusion, the effective components’content, accumulation amount and proportion of L. japonica are closely related to its development period, and the optimal harvest time is white bud stage.