| 农杆菌介导陆地棉转化和再生体系的研究进展 |
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| 引用本文： | 解晓红,张换样,李静,焦改丽,吴慎杰.农杆菌介导陆地棉转化和再生体系的研究进展[J].棉花学报,2011,23(4):379-384. |
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| 作者姓名： | 解晓红 张换样 李静 焦改丽 吴慎杰 |
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| 作者单位： | 山西省农科院棉花研究所,运城,044000 |
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| 基金项目： | 国家自然基金-科学部主任基金，国家转基因专项(2008ZX08005-004，山西省自然基金，山西省博士基金，山西省科技攻关项目 |
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| 摘 要： | 陆地棉通过体细胞胚胎发生的农杆菌介导转化体系的主要瓶颈在于:基因型限制、转化周期长、胚胎发生率低、易出现畸形胚等问题.针对这些问题,研究者对传统转化体系的各个环节进行了优化,并在拓展受体基因型、筛选高频再生品(种)系或株系、选择不同的组织或器官做受体和建立新的不依赖于组织培养和基因型限制的农杆菌转化体系等方面进行了探索...
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| 关 键 词： | 棉花 农杆菌介导转化 转化体系 再生体系 |
Advances of Agrobacterium-mediated Transformation and Regeneration System of Upland Cotton |
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| XIE Xiao-hong,ZHANG Huan-yang,LI Jing,JIAO gai-li,WU Shen-jie.Advances of Agrobacterium-mediated Transformation and Regeneration System of Upland Cotton[J].Cotton Science,2011,23(4):379-384. |
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| Authors: | XIE Xiao-hong ZHANG Huan-yang LI Jing JIAO gai-li WU Shen-jie |
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| Institution: | Cotton Research Institute of Shanxi Academy of Agricultural Sciences, Yuncheng, Shanxi 044000, China |
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| Abstract: | Advance of Agrobacterium-mediated transformation and regeneration system of cotton（Gossypium hirsutum L.） is summarized. Genotype-dependent, long transformation period, low frequency of embryogenesis, and high rates of abnormal embryos are main bottlenecks of Agrobacterium-mediated transformation of cotton via somatic embryogenesis. To solve these problems, the researchers optimized traditional transformation systems, expanded recipient genotypes, screened high-frequency regeneration lines, selected different organs or tissues as recipient explants, and established new methods of Agrobacterium-mediated cotton transformation which is tissue culture- and genotypes-independent. |
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| Keywords: | Agrobacterium mediated transformation transformation system regeneration system |
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