| 连香树再生体系的建立 |
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| 引用本文： | 张先云,袁秀云,马杰,袁丽洁.连香树再生体系的建立[J].北方园艺,2009(9). |
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| 作者姓名： | 张先云 袁秀云 马杰 袁丽洁 |
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| 作者单位： | 郑州师范高等专科学校,生物技术研究所,河南,郑州,450044;郑州师范高等专科学校,生物技术研究所,河南,郑州,450044;郑州师范高等专科学校,生物技术研究所,河南,郑州,450044;郑州师范高等专科学校,生物技术研究所,河南,郑州,450044 |
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| 基金项目： | 河南省科技攻关资助项目 |
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| 摘 要： | 对连香树进行无菌苗萌发和植株再生进行了研究.结果表明:以5～6月为最适取材季节;最佳外植体为当年生枝条顶芽下第3～4腋芽;腋芽诱导的适宜培养基为MS+BA1.0 mg/L+NAA 1.0 mg/L;适宜无菌播种的培养基为1/2MS+BA 0.5 mg/L+NAA 0.1 mg/L;适宜增殖培养基为MS+BA 0.5 mg/L+NAA 0.2 mg/L;适宜诱导愈伤的培养基为MS+BA 0.5 mg/L+NAA 0.5 mg/L;诱导分化的适宜培养基为MS+BA 1.0 mg/L+NAA 0.5 mg/L;生根培养基为1/2MS+NAA 0.5 mg/L.
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| 关 键 词： | 连香树 再生 快繁 |
Research on Tissue Culture and Regeneration of Cercidiphyllum japonicum |
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| ZHANG Xian-yun,YUAN Xiu-yun,MA Jie,YUAN Li-jie.Research on Tissue Culture and Regeneration of Cercidiphyllum japonicum[J].Northern Horticulture,2009(9). |
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| Authors: | ZHANG Xian-yun YUAN Xiu-yun MA Jie YUAN Li-jie |
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| Institution: | Institute of Biology Technology;Zhengzhou Teacher's College;Zhengzhou;Henan 450044;China |
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| Abstract: | In this paper the germination of seed and plant regeneration of Cercidiphyllum japonicum was researched.The results showed that it's optimum for sampling was in May and June;Third or forth bud in annual shoot were optimum explant;MS+BA 1.0 mg/L+NAA 1.0 mg/L was optimum to induce axillary bud;1/2MS+BA 0.5 mg/L+NAA 0.1 mg/L was suit to asepsis sowing;MS+BA 0.5 mg/L+NAA 0.2 mg/L was the optimum medium for reduplication;MS+BA 0.5 mg/L+NAA 0.5 mg/L was optimum to induce callus;MS+BA 1.0 mg/L+NAA 0.5 mg/L was opt... |
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| Keywords: | Cercidiphyllum japonicum Plant regeneration Intermediate propagation |
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