毒死蜱致2种作物中可溶性蛋白及相关酶的活性变化

为探讨农药"毒死蜱"与韭菜中解毒代谢酶的关系,本试验以毒死蜱喷施韭菜,研究了不同作用时间韭菜中可溶性蛋白质含量,谷胱甘肽-S-转移酶(GST)、谷胱甘肽过氧化物酶(GSH-PX)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(AKP)活性的变化动态并以小白菜与之对照。结果表明:韭菜和小白菜可溶性蛋白在受到毒死蜱作用时,其蛋白含量变化动态型式基本一致,呈立即升高随后降低又升高的趋势,并分别在14和5d恢复到正常水平;GST,GSH-PX的活性在韭菜中的变化不明显,在小白菜中程上升趋势;正常情况下2种蔬菜中ACP的活性是AKP的100倍左右,受胁迫时韭菜两磷酸酯酶变化不大,小白菜的两磷酸酯酶活性在作用后期迅速下降。毒死蜱在小白菜中的作用时间比韭菜短;小白菜的解毒代谢酶活性较韭菜高;小白菜能够更好的保护细胞膜结构并维护自身功能的完整。

Effects of chlorpyrifos stress on soluble proteins and somerelated metabolic enzyme activities in different crops

This paper is concerned the relationships between Chlorpyrifos and detoxification metabolic enzyme in leek.The contents of soluble proteins and activity dynamics of Glutathione-S-transferase (GST),glutathione peroxidase (GSH-PX),acid phosphatase (ACP),and alkaline phosphatase (AKP) was studied in leek at different time,using pakchoi as control.The results showed that 1) the changes of soluble proteins in leek was same as that of pakchoi,at a model of up-down-up following spraying Chlorpyrifos.2) The contents of soluble proteins increased to the normal levels after 14 and 5days.3) The activity of GST and GSH-PX in pakchoi changed more significantly than that in leek.4) The activity of ACP was usually 100 times as high as that of AKP.Activity of ACP,AKP in leek did not change obviously.However,activity of ACP,AKP in pakchoi reduced significantly in late period.Chlorpyrifos affected pakchoi for a short time.The detoxification enzyme activity in pakchoi was higher than that in leek.Cabbage had higher capacity to protect cell membrane structure and maintain its own function in integrity.