| 盐芥(Thellungiella halophila)DREB1E基因的克隆 |
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| 引用本文： | 韦善君,周宜君,徐小静,冯金朝.盐芥(Thellungiella halophila)DREB1E基因的克隆[J].华中农业大学学报,2008,27(2):177-181. |
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| 作者姓名： | 韦善君 周宜君 徐小静 冯金朝 |
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| 作者单位： | 中央民族大学生命与环境科学学院,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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面向21世纪教育振兴行动计划（985计划） |
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| 摘 要： | 以盐生植物盐芥为材料,依据拟南芥中DREB1E的序列信息设计引物,扩增出盐芥中DREB1E的部分序列,然后使用SMARTTMRACE等方法,从盐芥中克隆到全长的DREB1E cDNA序列.将这一基因命名为ThDREB1E.同源性分析结果表明,ThDREB1E与拟南芥DREB1E cDNA编码区的同源性为83.78%;根据基因核酸序列推测的氨基酸序列同源性为81.18%.
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| 关 键 词： | 盐芥 DERBlE基因 克隆 |
Cloning of DREB1E Gene from Thellungiella halophila |
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| WEI Shan-jun ZHOU Yi-jun XU Xiao-jing FENG Jin-chao.Cloning of DREB1E Gene from Thellungiella halophila[J].Journal of Huazhong Agricultural University,2008,27(2):177-181. |
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| Authors: | WEI Shan-jun ZHOU Yi-jun XU Xiao-jing FENG Jin-chao |
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| Institution: | College of Life and Environmental Sciences;Central University for Nationalities;Beijing 100081;China |
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| Abstract: | Dehydration responsive element binding 1E(DREB1E),induced to express in high-salt stress,is a member of CRT/DRE binding factors(CBFs)in Arabidopsis.A partial gene homologue to DERB1E was obtained from Thellungiella halophila using PCR-based technique with a pair of primers designed on the basis of the DREB1E gene of Arabidopsis.Then,the full length of the gene was cloned from Thellungiella halophila by SMARTTM RACE based on the sequence information of the partial gene sequence.It is designated as ThDERB1E.T... |
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| Keywords: | Thellungiella halophila DREB1E gene cloning |
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