| 大白菜抗霜霉病同源基因的筛选与亚克隆文库的构建 |
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| 引用本文： | 张宏,轩淑欣,马爱茹,申书兴,王彦华. 大白菜抗霜霉病同源基因的筛选与亚克隆文库的构建[J]. 河北农业大学学报, 2011, 34(3): 41-44 |
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| 作者姓名： | 张宏 轩淑欣 马爱茹 申书兴 王彦华 |
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| 作者单位： | 河北农业大学园艺学院,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金(30871713); 河北省自然科学基金(C2010000677,C2010000738) |
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| 摘 要： | 根据植物抗霜霉病基因保守区设计简并引物,利用三维PCR方法对大白菜(Brassica campestris ssp.pekiensis)‘85-1'BAC文库进行筛选,从19 200个BAC克隆中筛选到含有目的基因的10个阳性克隆.利用HindⅡ对其中的94一G-17克隆进行酶切,回收1～5 kb的DNA片段并连接到载...
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| 关 键 词： | 大白菜 霜霉病 抗病同源基因 BAC克隆 亚克隆文库 |
Identification of downy mildew resistance analog gene and construction of sub-clone library on Chinese cabbage |
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| ZHANG Hong,XUAN Shu-xin,MA Ai-ru,SHEN Shu-xing,WANG Yan-hua. Identification of downy mildew resistance analog gene and construction of sub-clone library on Chinese cabbage[J]. Journal of Agricultural University of Hebei, 2011, 34(3): 41-44 |
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| Authors: | ZHANG Hong XUAN Shu-xin MA Ai-ru SHEN Shu-xing WANG Yan-hua |
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| Affiliation: | ZHANG Hong,XUAN Shu-xin,MA Ai-ru,SHEN Shu-xing,WANG Yan-hua(College of Horticulture,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | The degenerated primers were designed according to the conservative domain of most disease resistant genes of downy mildew in plants.Screening the Chinese cabbage '85-1' BAC library through three steps of PCR,10 positive clones containing target gene from 19200 BAC clones were obtained.Digesting the BAC clone 94-G-17 with HindⅡ,1-5 kb fragments were collected.Then,these fragments were ligated on the pUCHindⅡ/BAP vectors,and a sub-clone library containing 6 200 clones were obtained.By electrophoresis detection,the insert fragments were from 1 kb to 5 kb.The sub-clone library could be used for obtaining the complete sequence of downy mildew resistance gene in Chinese cabbage. |
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| Keywords: | Chinese cabbage downy mildew resistance analog gene BAC clone sub-clone library |
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