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昆虫病原线虫抗干燥能力及其对梨小食心虫致死能力研究
引用本文:李宁,王志刚,阎爱华,王勤英,黄大庄,毕拥国,马向超.昆虫病原线虫抗干燥能力及其对梨小食心虫致死能力研究[J].河北农业大学学报,2011,34(2):90-93.
作者姓名:李宁  王志刚  阎爱华  王勤英  黄大庄  毕拥国  马向超
作者单位:1. 河北农业大学林学院,河北保定,071001
2. 河北农业大学植物保护学院,河北保定,071001
基金项目:北方果树综合防控技术研究与示范推广(201103024)
摘    要:为筛选出抗干燥能力较强的昆虫病原线虫,采用小空间控制饱和盐液法测定了10个昆虫病原线虫品系的抗干燥能力,结果表明:不同昆虫病原线虫品系抗干燥能力不同.在25℃、相对湿度50.5%的小空间控制器中处理9 h后,Steinernema carpocapsae GA品系的存活率最高,达到75.1%,且复苏后线虫对大蜡螟致死率...

关 键 词:昆虫病原线虫  抗干燥  梨小食心虫

Anti-desiccation ability of several entomopathogenic nematode strains and their pathogenity to Grapholitha molesta
LI Ning,WANG Zhi-gang,YAN Ai-hua,WANG Qin-ying,HUANG Da-zhuang,BI Yong-guo,MA Xiang-chao.Anti-desiccation ability of several entomopathogenic nematode strains and their pathogenity to Grapholitha molesta[J].Journal of Agricultural University of Hebei,2011,34(2):90-93.
Authors:LI Ning  WANG Zhi-gang  YAN Ai-hua  WANG Qin-ying  HUANG Da-zhuang  BI Yong-guo  MA Xiang-chao
Institution:LI Ning1,WANG Zhi-gang1,YAN Ai-hua1,WANG Qin-ying2,HUANG Da-zhuang1,BI Yong-guo1,MA Xiang-chao1(1.College of Forestry,Agricultural University of Hebei,Baoding 071001,China,2.College of Plant Protection,China)
Abstract:In order to screen out the higher anti-desiccation ability of entomopathogenic nematodes,the anti-desiccation ability of 10 entomopathogenic nematodes strains were evaluated in a desiccator containing saturated salt solution.The results showed that their anti-desiccation ability varied between different nematode strains.Steinernema had a higher survival rate when treated at 25 ℃ and RH of 50.5% for 9 h than Heterorabditis,S.carpocapsae GA has the highest survival rate of 75.1%.And the mortality rate to mellonella of recovery nematodes is 100%.Heterorhabditis sp.CZ and XH-9 strain survival rate is 0.By using several higher anti-desiccation ability strains,the pathogenity of the entomopathogenic nematode to the Grapholitha molesta was also determined.The results indicated that ZH has strong virulence to Grapholitha molesta,and LT50 is 2.46 d.
Keywords:entomopathogenic nematodes  anti-desiccation ability  Grapholitha molesta  
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