Analysis of the Resistance Gene Analogue for Rice Cultivars in Yunnan Province |
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| Authors: | SUN Yan WANG Yun-yue HE Yue-qiu FAN Jing-hua CHEN Jian-bin ZHU You-yong |
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| Institution: | 1. Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201 , P. R. China;College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201 , P. R. China
2. Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201 , P. R. China |
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| Abstract: | Genetic diversity of commercial and local rice cultivars in Yunnan Province was studied using the resistance gene analogue (RGA) based on resistance gene conserved sequences. The RGA analysis of 137cultivars was conducted by PCR amplification using three primers, i.e. S1/AS3, XLRR for/XLRR rev, and Pto-kin1/Pto-kin2, respectively. The results showed that both Indica and Japonica cultivars were genetically highly diverse. All cultivars were divided into 3 lineages according to the DNA band data at 96% dissimilarity,and into 20 lineages at 60% dissimilarity. The lineages were related to their genetic background and blast disease resistance with only a few exceptions. The RGA data can be useful in rice production by mixed-planting of different cultivars in the field and breeding of resistance cultivars by selecting different parental cultivars with great genetic diversity. |
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| Keywords: | Rice Resistance gene analogue Blast disease resistance |
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