| 猪瘟病毒石门株E2基因的RT-PCR克隆及鉴定 |
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| 引用本文： | 王宁,张楚瑜,朱燕,付烈振.猪瘟病毒石门株E2基因的RT-PCR克隆及鉴定[J].中国兽医杂志,1999,25(1):8-9. |
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| 作者姓名： | 王宁 张楚瑜 朱燕 付烈振 |
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| 作者单位： | 武汉大学生科院动物病毒室,武汉,430072 |
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| 基金项目： | 国家攀登计划85－44－01－03资助 |
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| 摘 要： | 本文采用ＲＴ－ＰＣＲ技术从感染猪血中成功扩增了我国猪瘟病毒强毒石门株Ｅ２基因，大小为１１８４ｂｐ，与预期大小一致。经巢式ＰＣＲ和酶切鉴定证实所扩增的片段为Ｅ２基因特异性片段。将扩增的Ｅ２基因克隆到Ｐ＾ＧＥＭ－Ｔ载体，采用限制性内切酶鉴定和ＰＣＲ技术了阳性重组子。
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| 关 键 词： | 猪瘟病毒 石门株 RT－PCR E2基因 克隆 |
Amplification and cloning of E2 gene of Classical swine fever virus shimen strain |
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| Wang Ning,Zhang Chuyu,Zhu yan,Fu Liezhen.Amplification and cloning of E2 gene of Classical swine fever virus shimen strain[J].Chinese Journal of Veterinary Medicine,1999,25(1):8-9. |
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| Authors: | Wang Ning Zhang Chuyu Zhu yan Fu Liezhen |
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| Abstract: | The E2 gene fragment of classical swine fever virus shimen strain was amplified from RNA extracted from blood of infected pig with PT PCR method.The amplified E2 fragment was 1184bp in length.The specificity of the amplified E2 framgent was confirmed by nested PCR and internal digest of restriction endonucleases.This unmodified E2 fragment was directly inserted into pGEM T vector.The recombinant plasmid DNAs of positive colonies were selected by digestion of Restriction endonucleases and PCR. |
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| Keywords: | Classical swine fever virus shimen strain RT PCR E2 gene clone |
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