| 夏橙果实离层中伤害诱导基因的克隆与表达分析 |
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| 引用本文： | 杨雪莲,张凌云,王志卫,黄勇,闫树堂,钟广炎.夏橙果实离层中伤害诱导基因的克隆与表达分析[J].果树学报,2010(6). |
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| 作者姓名： | 杨雪莲 张凌云 王志卫 黄勇 闫树堂 钟广炎 |
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| 作者单位： | 西南大学;贵州大学;中国农业科学院柑桔研究所; |
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| 基金项目： | 重庆市科技攻关计划项目(CSTC,2007AA1018); 国家柑橘产业技术体系支持项目 |
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| 摘 要： | 从乙烯处理的伏令夏橙果实离层中克隆出4个伤害诱导基因(CsWIP1、CsWIP2、CsWIP3、CsWIP4)。通过RT-PCR、RACE等克隆技术分别获得了CsWIP1、CsWIP2、CsWIP3和CsWIP4的全长cDNA。测序结果表明CsWIP1、CsWIP2、CsWIP3和CsWIP4全长cDNA分别为588、549、412和629bp,开放阅读框分别编码98、95、83和88个氨基酸,结构模拟显示这些蛋白在C-端和N-端各有一个α-螺旋,中间是一个富丝氨酸域。与其他植物伤害诱导蛋白序列比对结果显示,CsWIP家族蛋白分为2类,CsWIP1、CsWIP2和CsWIP3与玉米WIP家族中NP_001148450.1、NP_001151919.1、NP_001151459.1、NP_001147041.1和拟南芥NP_192765.1、NP_849355.1、NP_179023.1聚成一类;CsWIP4与葡萄的AY159560.1聚成一类。通过实时定量PCR检测这些基因在乙烯处理下果实离层中的表达情况,结果显示CsWIP1和CsWIP2受乙烯强烈诱导,CsWIP3和CsWIP4几乎不受乙烯调控。
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| 关 键 词： | 夏橙 伤害诱导蛋白 基因家族 克隆 乙烯诱导 |
Cloning of wound-induced protein genes from Citrus sinensis cv. Valencia and analysis of their expression patterns in fruit calyx abscission zone |
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| YANG Xue-lian,ZHANG Ling-yun,WANG Zhi-wei,HUANG Yong,YAN Shu-tang,ZHONG Guang-yan.Cloning of wound-induced protein genes from Citrus sinensis cv. Valencia and analysis of their expression patterns in fruit calyx abscission zone[J].Journal of Fruit Science,2010(6). |
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| Authors: | YANG Xue-lian ZHANG Ling-yun WANG Zhi-wei HUANG Yong YAN Shu-tang ZHONG Guang-yan |
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| Institution: | YANG Xue-lian1,3,ZHANG Ling-yun1,WANG Zhi-wei1,HUANG Yong1,YAN Shu-tang1,2,ZHONG Guang-yan1,2 (1Southwest University,Beibei,Chongqing 400715 China,2Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 400712 China,3Guizhou University,Guiyang,Guizhou 550025 China) |
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| Abstract: | Four wound-induced genes,CsWIP1,CsWIP2,CsWIP3 and CsWIP4,were cloned from ethylene-treated abscission zones ( AZ-C ) of citrus (Citrus sinensis cv.Olinda valencia ).Their full-length cDNAs were obtained by using RT-PCR and RACE.The full-length cDNAs of CsWIP1,CsWIP2,CsWIP3 and CsWIP4 were 588,549,412 and 629 bp and the corresponding ORFs would encode 98,95,83 and 88 amino-acid residues,respectively.Structural simulations showed that the deduced proteins possessed two -helices located separately at Nand C-te... |
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| Keywords: | Citrus sinensis cv Valencia Wound-induced protein (WIP) Gene family Cloning Ethylene induction |
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