安徽地区禽源沙门氏菌分离鉴定与耐药性研究

为了解安徽地区禽源沙门氏菌的流行菌株和耐药情况，本研究通过培养特性、镜检、生化试验、PCR和血清型鉴定等，对疑似发病禽的116份病料进行沙门氏菌分离鉴定，并采用Kindy-Bauer法对分离菌株进行耐药性分析。试验共分离到42株禽源沙门氏菌，其中包含5个大群11种血清型。B群30株、A群2株、D群7株、C₁群2株、C₂群1株，其中以B群鼠伤寒沙门氏菌为优势菌株。42株分离菌对阿米卡星、左氧氟沙星和环丙沙星的耐药率均低于20%，而对氨苄西林、链霉素、呋喃唑酮、大观霉素、复方新诺明和多西环素的耐药率达50%以上，其中对氨苄西林的耐药率达97.62%，且41株分离菌株存在多重耐药。结果表明，目前安徽地区禽源沙门氏菌优势流行株是鼠伤寒沙门氏菌，流行菌株呈现较严重的耐药性。本研究为指导临床用药及禽源沙门氏菌的综合防制提供数据支撑。

Isolation,Identification and Drug Resistance Analysis of Avian Salmonella in Anhui

In order to investigate the situation of predominant strain and antibiotic resistance of avian Salmonella in Anhui province, 42 strains Salmonella were isolated and identified from 116 suspected samples by culture characteristics, microscopic examination, biochemical test, PCR and serological identification,then drug resistance was analyzed by Kindy-Bauer method. There were 5 groups of 11 serotypes in 42 strains Salmonella, of which 30 strains belonged to B serogroup, 2 strains to A serogroup, 7 strains to D serogroup, 2 strains to C₁ serogroup, 1 strain to C₂ serogroup, and Salmonella Typhimurium of B serogroup were the predominant serotypes. All Salmonella isolates resistant rates to amikacin, levofloxacin and ciprofloxacin were less than 20%, and the resistance rates to ampicillin, streptomycin, furazolidone, spectinomycin, cotrimoxazole and doxycycline were more than 50%, while resistance rate to ampicillin was 97.62%, and 41 isolates had multiple drug resistance. The results showed that Salmonella Typhimurium was the predominant serotypes of avian Salmonella in Anhui, and isolates showed multiple drug resistance. This study would provide the data basis for the comprehensive prevention and treatment of clinical medicine of avian Salmonella.