| 间接Dot—ELISA检测EDS—76病毒抗原的研究 |
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| 引用本文： | 张晓根,唐光武.间接Dot—ELISA检测EDS—76病毒抗原的研究[J].郑州牧业工程高等专科学校学报,2000,20(1):1-2,38. |
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| 作者姓名： | 张晓根 唐光武 |
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| 作者单位： | 郑州牧业工程高等专科学校,郑州 |
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| 摘 要： | 本研究成功地建立了间接酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）用于检测ＥＤＳ－７６病毒抗原的标准化程序。对纯化ＥＤＳ－７６病毒抗原的最低检出量为１ｎｇ／Ｄｏｔ，其敏感性约为ＨＡ的１５．６倍。ＥＤＳ－７６阳性鸡血清特异性阻断试验及交叉反应试验证明，该法对ＥＤＳ－７６病毒抗原的检测具有特异性。试验结果表明，间接Ｄｏｔ－ＥＬＩＳＡ检测ＥＤＳ－７６病毒抗原，具有敏感性高、特异性强、重复性好、经济、方便、快速等优点，适合于大规模样本的检测。
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| 关 键 词： | EDS－76病毒 Dot－ELISA 抗原检测 鸡 咸蛋综合症 |
Study on the use of indirect Dot-ELISA in examining EDS 76 virus antigens |
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| Zhang Xiaogen,Tang Guangwu,Liu Xingyou and Wang Degang.Study on the use of indirect Dot-ELISA in examining EDS 76 virus antigens[J].Journal of Zhengzhou College of Animal Husbandry Engineering,2000,20(1):1-2,38. |
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| Authors: | Zhang Xiaogen Tang Guangwu Liu Xingyou and Wang Degang |
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| Abstract: | A standard procedure was successfully established to examine EDS 76 virus antigens with the indirect dot enzyme linked immunosorbent assay (Dot ELISA).Pure EDS 76 virus antigens were detectable at a minimum of l ng/dot, and 15.6 times as sensitive as HA. Distinctive obstruction and cross response tests with EDS 76 positive chicken serum indicated that this method was specific to EDS 76 virus antigens. It was concluded that examination of EDS 76 virus antigens with the indirect Dot ELISA is highly sensitive, specfic, repeatable, economical, convenient and quick, and applicable to examination of large sized samples. |
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| Keywords: | EDS-76 virus Dot ELISA antigen examination |
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