嗜热毛壳菌纤维素酶对小尾寒羊瘤胃代谢的影响

本试验研究不同水平嗜热毛壳菌纤维素酶对小尾寒羊瘤胃代谢的影响。试验动物为4只安装有永久性瘤胃瘘管的成年小尾寒羊公羊(平均体重为45 kg),采用4×4拉丁方试验设计,在基础日粮中分别添加0％、0．3％、0．6％和0．9％4个水平酶制剂,采集瘤胃液测pH值、氨氮浓度和挥发性脂肪酸(VFA)浓度。试验结果表明:各组试羊瘤胃液平均氨氮浓度的变化范围为10．01-12．80 mg/dL,各组之间相同时间点差异不显著(P>0．05)。瘤胃液平均pH值在6．50-6．80范围内变动,试验组pH值低于对照组(P<0．05),以0．6％水平较好。各组试羊瘤胃乙酸、丙酸及总 VFA浓度的变化规律基本相同,即喂料后逐渐上升,其中乙酸和总VFA浓度在2 h后达到最高点,丙酸浓度在4 h 达到最高点,随后平稳下降于饲喂前降至最低点,再次采食后又重复出现此规律。试验组瘤胃乙酸和总VFA浓度显著或极显著高于对照组(P<0．05或P<0．01),以0．6％水平组最高。

Rumen Fermentation of Sheep Under Different Cellulase Supplementing Level

Four grown small tail Han-sheep with average weight of 45 kg fitted with permanent ruminal fistulae were used to study the effects of C. thermophile cellulase on rumen fermentation. The experiments were performed in 4x4 Latin square design. The experimental diets consist of the basal diet supplemented with 0%,0.3%,0.6%,0.9% of four levels of C. thermophile cellulase.pH values, ammonia-N and VFA concentrations of rumen fluid samples were measured. The results indicated that: the ruminal ammonia-N concentration of the four levels had no significant effect (P> 0.05). The pH values of rumen fluid of the test groups were lower than that of the control group (P < 0.05). But the acetate and VFA concentrations of rumen fluid were significantly higher than that of the control (P< 0.05 or P< 0.01). The level of 0.6% was the highest.