| 大蒜抗根结线虫内生细菌的筛选及盆栽防效试验 |
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| 引用本文： | 黄开伟,李敏敏,史倩倩.大蒜抗根结线虫内生细菌的筛选及盆栽防效试验[J].农产品加工．学刊,2020(4):20-21,25. |
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| 作者姓名： | 黄开伟 李敏敏 史倩倩 |
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| 作者单位： | 青岛农业大学植物医学学院;山东省植物保护总站 |
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| 基金项目： | 青岛农业大学2019年大学生创新训练项目（S201910435055）;青岛农业大学高层次人才科研启动基金项目（1119011） |
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| 摘 要： | 利用稀释涂布平板法,从受根结线虫为害的大蒜根须组织研磨液中筛选对根结线虫有明显抑杀效果的内生生防细菌。结果表明,在盆栽试验中,菌株S1-1对根结线法的杀线虫由活性为90.8%。通过盆栽试验验证生防菌株的防效,菌株S1-1防效为74.86%。结合菌株形态特征、分子生物学和生理生化特性,将菌株S1-1鉴定为短小芽孢杆菌(Bacillus pumilus)。
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| 关 键 词： | 根结线虫 生防细菌 筛选 芽孢杆菌 试验 |
Screening and Identification of Bacteria from Garlic Root Against Root-knot Nematode and Pot Experiment |
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| HUANG Kaiwei,LI Minmin,SHI Qianqian.Screening and Identification of Bacteria from Garlic Root Against Root-knot Nematode and Pot Experiment[J].Nongchanpin Jlagong.Xuekan,2020(4):20-21,25. |
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| Authors: | HUANG Kaiwei LI Minmin SHI Qianqian |
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| Institution: | (College of Plant Health and Medicine,Qingdao Agricultural University,Key Laboratory of Integrated Crop Pest Management of Shandong Province,Qingdao,Shandong 266109,China;Plant Protection Station of Shandong Province,Jinan,Shandong 250100,China) |
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| Abstract: | The screening and identification of biocontrol bacteria from garlic root against root-knot nematode was performed using dilution methode.The results showed that the nematicidal activity of strain S1-1 against Meloidogyne incognita was 90.8%,and biocontrol effect of strain S1-1 was up to 74.86%against Meloidogyne incognita in pot experiment.Combined its morphlogicul characteristics,morphological characteristics and biochemical characteristics,Strain S1-1 was identified as Bacillus pumilus. |
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| Keywords: | root-knot nematode biocontrol bacteria screening Bacillus spp experiment |
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