铅与维生素C对小鼠肝脏脂质过氧化的影响

以五周龄昆白系小鼠为试验动物，在饲喂小鼠普通日粮基础上每天灌喂一定量铅（Pb）、维生素C（Vc）、Vc+Pb，Vc用量200mg/kg，Pb四个浓度分别为1.2g/L 、0.4g/L、0.13g/L、0.043g/L，饲喂期三周，检测小鼠肝脏中铅与丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性变化。结果表明：铅在小鼠肝脏中大量蓄集，并引起MDA含量增加；加Pb组与空白对照组相比SOD酶活性降低，且随着铅浓度的降低而升高；CAT活性测定显示： Pb1、Pb2组与空白对照组相比差异显著（p<0.05），Pb3组差异极显著(p<0.01)，除Vc+Pb4组与Pb4组相比差异不显著(P>0.05)外，其他Vc+Pb组与其对应加Pb组比较，均差异显著（p<0.05）；POD活性测定表明：Vc组与空白组相比差异极显著(p<0.01)，Pb1、Pb3组与空白组比较差异显著（p<0.05），Pb2、Pb4与空白组比较差异极显著(p<0.01)，除Vc+Pb1组与Pb1组差异不显著(P>0.05)，其他Vc加Pb组与单独加Pb组相比差异极显著(p<0.01)。因此，Pb可增强小鼠肝脏脂质过氧化，而Vc可减轻铅引起的过氧化损伤

Effects of Plumbum and Vitamin C on Liver Lipid Peroxides Activity in Mice

The experiment adopt five weeks old healthy Kunbai mice as experimental animal.The mice were fed the common fodder and given plumbum(Pb)，vitamin C(Vc) and the mixtures of these two things.The dosage of the Vc is 200mg/kg .The dosage of Pb had four grads 1.2g/L、0.4g/L、0.13g/L、0.043g/L .The experiment lasts three weeks.Then detect the Pb MDA content and SOD POD CAT activity .The results show that the plumbum accumulated in liver largely,and the content of MDA increased.The activity of SOD in every group lowered significantly (P<0.05) compared with the control group,and increased with the fall of Pb’s concentration. We mensurated the activity of CAT,the mensuration displayed that the Pb1、Pb2 group had significant difference（p<0.05）to compared with the control group,and the Pb3 had significant difference(p<0.01). At the same time, except the Vc+Pb4 group had no significant difference(p>0.05),the rest group had significant difference (p<0.05)compared with their corresponding groups which only add Pb. The mensuration of POD activity showed : compared with the control group,the Vc group had significant difference(p<0.01),the Pb1、Pb3 group had significant difference（p<0.05）.Compared with their corresponding groups which only add Pb,the Vc+Pb1 group had no significant difference（p>0.05）, the rest Vc+Pb group compared with their corresponding groups which only add Pb had significant difference(p<0.01). The the result of experiment indicated that the plumbum can intensify the lipid peroxidation,but Vc can lighten the scathe caused by lipid peroxidation.