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| 扬稻6号差异表达基因与杂种优势关系分析 | |
| 引用本文： | 张洪熙,戴正元,陈建民,肖宁,李爱宏,潘存红,刘广青,周长海,夏广宏.扬稻6号差异表达基因与杂种优势关系分析[J].上海交通大学学报(农业科学版),2008,26(6):560-566. |
| 作者姓名： | 张洪熙 戴正元 陈建民 肖宁 李爱宏 潘存红 刘广青 周长海 夏广宏 |
| 作者单位： | 1. 江苏里下河地区农业科学研究所,国家水稻改良中心南京分中心,扬州,225007 2. 扬州大学生物科学与技术学院,扬州,225002 3. 江苏里下河地区农业科学研究所,国家水稻改良中心南京分中心,扬州,225007;扬州大学生物科学与技术学院,扬州,225002 |
| 基金项目： | 国家科技攻关项目 ， 国家高技术研究发展计划(863计划) ， 江苏省高技术研究发展计划项目 |
| 摘 要： | 为探讨扬稻6号的杂种优势分子机理，以扬稻6号、特膏和明恢63配制的强、中、弱优势组合及其亲本为材料，利用DD-PCR技术。分析剑叶和幼穗的基因差异表达类型与主要农艺性状的杂种表现及杂种优势的关系。结果表明：千粒重与剑叶的DMP2（F1和父本表达的条带）和幼穗的UNP1（母本特异表达的条带）、UMONO（F1和双亲都沉默的条带）呈显著正相关，而与幼穗的UNF1（F1特异表达的条带）和MONO（F1和双亲都表达的条带）呈显著负相关。在超亲优势方面千粒重与幼穗的UNF1呈显著负相关。推测杂种优势可能受父本基因型影响较大。此外，分别克隆强、弱优势F1闻差异表达的条带，反Southem鉴定出40个差异表达基因。涉及光合作用、生物运输、逆境应答、转录调控、信号转导、糖代谢、氨基酸代谢等。 |
| 关 键 词： | 扬稻6号 杂种优势 基因差异表达 |
Analysis on Relationship between Differential Gene Expression and Heterosis in Yangdao 6 |
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| ZHANG Hong-xi,DAI Zheng-yuan,CHEN Jian-min,XIAO Ning,LI Ai-hong,PAN Cun-hong,LIU Guang-qing,ZHOU Chang-hai,XIA Guang-hong.Analysis on Relationship between Differential Gene Expression and Heterosis in Yangdao 6[J].Journal of Shanghai Jiaotong University (Agricultural Science),2008,26(6):560-566. | |
| Authors: | ZHANG Hong-xi DAI Zheng-yuan CHEN Jian-min XIAO Ning LI Ai-hong PAN Cun-hong LIU Guang-qing ZHOU Chang-hai XIA Guang-hong |
| Institution: | ZHANG Hong-xi1,DAI Zheng-yuan1,CHEN Jian-min2,XIAO Ning1,2,LI Ai-hong1,PAN Cun-hong1,LIU Guang-qing1,ZHOU Chang-hai1,XIA Guang-hong1(1.Lixiahe Region Agricultural Scientific Researcher Institute of Jiangsu Province / Nanjing Sub-center of National Rice Improvement Center,Yangzhou 225007,2.College of Bioscience , Bio-Technolog of Yangzhou University,Yangzhou 225000,China) |
| Abstract: | To understand the molecular mechanism of rice heterosis,differential gene expression patterns in flag leaves and young panicle of high-heterosis crosses with Yangdao 6 as male parent,middle-heterosis crosses with Teqing as male parent and low-het-erosis crosses with Minghui 63 male parent were analyzed by DD-PCR.The correlation analysis of various differential gene expression patterns with the performance and heterosis of main rice agronomic traits was carried out.The main results are as follow:The 1000-gra... |
| Keywords: | Yangdao 6 heterosis differential gene expression |
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