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大花蕙兰组织培养的研究
引用本文:王慧瑜,张晓申,杨录军,赵海红.大花蕙兰组织培养的研究[J].河北林果研究,2006,21(3):278-280.
作者姓名:王慧瑜  张晓申  杨录军  赵海红
作者单位:郑州市农林科学研究所,生物技术中心,河南,郑州,450005
摘    要:对大花蕙兰茎尖进行了组织培养研究。试验结果表明:原球茎诱导、增殖和生根培养基分别是MS 6-BA 1.0 mg/L NAA 0.1 mg/L 香蕉汁100 g/L 活性炭0.5 g/L,MS 6-BA 1.5 mg/L NAA 0.5 mg/L 香蕉汁130 g/L 活性炭1.0 g/L,1/2 MS 6-BA 0.1 mg/LAA 0.5 mg/L 香蕉汁150 g/L 活性炭1.5 g/L。

关 键 词:大花蕙兰  组织培养  原球茎
文章编号:1007-4961(2006)03-0278-03
修稿时间:2005年10月11

Studies on tissue culture of Cymbidum
WANG Hui-yu,ZHANG Xiao-shen,YANG Lu-jun,ZHAO Hai-hong.Studies on tissue culture of Cymbidum[J].Hebei Journal of Forestry and Orchard Research,2006,21(3):278-280.
Authors:WANG Hui-yu  ZHANG Xiao-shen  YANG Lu-jun  ZHAO Hai-hong
Abstract:The tissue culture of Cymbidum were studied by using shoot-tip.The results showed that the explants cultured on MS 6-BA 1.0 mg/L NAA 0.1 mg/L banana 100 g/L carbon 0.5 g/L,and MS 6-BA 1.5 mg/L NAA 0.5 mg/L banana 130 g/L carbon 1.0 g/L,and 1/2 MS 6-BA 0.1 mg/L NAA 0.5 mg/L banana 150 g/L carbon 1.5 g/L were suitable for inducing PLBs,multiplicating PLBs and rooting of microshoots,respectirely.
Keywords:Cymbidum  tissue culture  protocorm-like-bodies(PLBs)  
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