| 灵芝漆酶催化活性艳色蓝X-BR脱色条件的研究 |
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| 引用本文： | 韩业昊,张超,凌庆枝.灵芝漆酶催化活性艳色蓝X-BR脱色条件的研究[J].安徽农业科学,2014(26):8875-8877. |
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| 作者姓名： | 韩业昊 张超 凌庆枝 |
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| 作者单位： | 安徽理工大学医学院,安徽淮南,232001;浙江医药高等专科学校,浙江宁波,315100 |
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| 基金项目： | 浙江省科技厅公益技术研究社会发展项目 |
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| 摘 要： | 目的]研究灵芝（Ganoderma lucidum）固体发酵所得漆酶粗酶液对蒽醌染料进行脱色的条件.方法]分别研究不同的pH、染料浓度、温度、酶用量、反应时间等条件对活性艳色蓝X-BR染料脱色的影响.结果]脱色的适宜条件为：在pH 3.5、40℃、60 U/ml灵芝漆酶、200 mg/L染料浓度的条件下反应5h,活性艳色蓝X-BR染料脱色率可达49％.结论]灵芝固体发酵的漆酶粗酶液在一定条件下对蒽醌染料活性艳色蓝X-BR具有较好的脱色效果.
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| 关 键 词： | 灵芝漆酶 活性艳色蓝X-BR 脱色 |
Study on the Decolorized Condition on Reactive Brilliant Blue X-BR by Laccase of Ganoderma lucidum |
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| HAN Ye-hao,ZHANG Chao,LING Qing-zhi.Study on the Decolorized Condition on Reactive Brilliant Blue X-BR by Laccase of Ganoderma lucidum[J].Journal of Anhui Agricultural Sciences,2014(26):8875-8877. |
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| Authors: | HAN Ye-hao ZHANG Chao LING Qing-zhi |
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| Institution: | HAN Ye-hao;LING Qing-zhi;Medical College Anhui University of Science and Technology;Zhejiang Pharmaceutical College; |
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| Abstract: | Objective ] Decolorization of anthraquinone dye with laccase from C, anoderma lucidum was studied. Method] Effects of different pH, dye concentrations, temperature, enzyme dosage, reaction time on decolorization of Reactive Brilliant Blue X-BR were studied. Result ] The optimal conditions of decolorization are： pH 3.5,40 ℃, 60 U/ml Ganoderma laccase and 200 mg/L dye concentration, respectively. Un- der the above conditions, the decolorization rate of Reactive Brilliant Blue X-BR was 49% after 5 h catalyzation with the laccase from G. luci- dum. Conclusion] Ganoderma laccase under optimal conditions for Reactive Brilliant Blue X-BR has a decolorization effect. |
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| Keywords: | Ganoderma lucidum Reactive Brilliant Blue X-BR Decolorization |
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