| 黑曲霉发酵麦麸生产β-葡萄糖苷酶的培养基组成的优化 |
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| 引用本文： | 杨正,潘利华,罗建平.黑曲霉发酵麦麸生产β-葡萄糖苷酶的培养基组成的优化[J].农产品加工．学刊,2012(12):36-41. |
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| 作者姓名： | 杨正 潘利华 罗建平 |
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| 作者单位： | 合肥工业大学;安徽化工学校化学系 |
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| 摘 要： | 以黑曲霉为发酵菌株,研究其β-葡萄糖苷酶液体发酵的最佳培养基组成。通过对不同碳源、氮源、磷酸盐、金属离子、氨基酸、表面活性剂等单因素研究,发现麦麸、(NH_4)_2SO_4和KH_2PO_4对产酶有促进作用。3因素二次通用旋转组合设计试验得到该菌株产β-葡萄糖苷酶的优化培养基组成为:麦麸28.4 g/L,(NH_4)_2SO_41.2 g/L,KH_2PO_42.0 g/L,发酵液β-葡萄糖苷酶酶活达到(1 493.90±12.09)U/mL。
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| 关 键 词： | 黑曲霉 β-葡萄糖苷酶 液体发酵 培养基组成 |
Effect of Media Composition on Production ofβ-Glucosidase by A spergillus niger with Wheat Bran |
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| YANG Zheng,PAN Li-hua,LUO Jian-ping.Effect of Media Composition on Production ofβ-Glucosidase by A spergillus niger with Wheat Bran[J].Nongchanpin Jlagong.Xuekan,2012(12):36-41. |
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| Authors: | YANG Zheng PAN Li-hua LUO Jian-ping |
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| Institution: | 1 (1.Hefei University of Technology,Hefei,Anhui 230009,China; 2.Chemistry Department,School of Physics and Electrical Engineering,Anqing,Anhui 246005,China) |
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| Abstract: | The optimum media compositions for theβ-glucosidase production from Aspergillus niger are studied.The effects of carbon source,nitrogen source,phosphate source,metal salts,amino acid and surfactant on theβ-glucosidase production are investigated.The results show that wheat bran,(NH_4)_2SO_4 and KH_2PO_4 have stimulatory effect onβ-glucosidase secretion. Theβ-glucosidase activity reaches(1 493.90±12.09) U/mL under the optimum fermentation media based on the three-factor second order rotation combination experimental design. |
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| Keywords: | Aspergillus niger β-glucosidase submerge fermentation media composition |
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