不同小麦品种成熟胚愈伤组织的培养研究

目的]通过对河北省小麦品种的种子进行组织培养,探索适合河北省栽培小麦品种的培养条件,为河北省小麦的遗传改良工作奠定基础。方法]对4种不同小麦品种的成熟胚进行离体培养,研究影响小麦愈伤组织诱导的因素。结果]结果表明:将成熟胚盾片向上放置可明显提高愈伤组织的诱导率;利用2 mg/L 2,4-D并添加水解酪蛋白,可使不同品种小麦愈伤组织得出愈率均高达80%;此外,不同品种的出愈率随着2,4-D浓度的升高存在较大差别。结论]该研究探索影响小麦成熟胚愈伤组织诱导的因素,建立一套合适的植株再生体系,为加强我国小麦的遗传转化奠定了基础。

Study on the Culture of Callus from Mature Embryos of Different Wheat Varieties

Objective] The seeds of wheat varieties of Hebei province were conducted for tissue culture to explore the culture condition for wheat cultivars of Hebei province and lay the base for genetic improvement of Hebei province.Method] The mature embryos from 4 kinds of wheat cultivars were cultured in vitro to study the factors influencing wheat callus induction.Result] Placing upward the scutellum of mature embryos could increase the callus induction rate obviously.The callus induction rates in different cultivars all reached as high as 80% by using 2 mg/L 2,4-D and adding hydrolisis casein.Callus induction of different cultivars were significantly different with the increment of 2,4-D concentration.Conclusion] this research explored the factors influencing callus induction of wheat mature embryos and established a set of suitable plant regeneration system,which lay the base for strengthening wheat genetic transformation in China.