| 独花兰组织培养研究简 |
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| 引用本文： | 刘国顺,杨丽,董卉卉,哈登龙,琚煜熙,王晶,马冠男. 独花兰组织培养研究简[J]. 安徽农业科学, 2013, 0(22): 9195-9196 |
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| 作者姓名： | 刘国顺 杨丽 董卉卉 哈登龙 琚煜熙 王晶 马冠男 |
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| 作者单位： | [1]信阳市林业科学研究所,河南信阳464031 [2]鸡公山国家级自然保护区管理局,河南信阳464133 [3]信阳农林学院,河南信阳464000 |
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| 基金项目： | 信阳市科技计划项目（KJGG1110）. |
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| 摘 要： | [目的]研究独兰花的组织培养方法.[方法]以独兰花假鳞茎为外植体,以1/2MS ＋0.5 mg/L 6-BA ＋0.05 mg/L NAA为芽诱导培养基,以1/2MS＋ 1.5 mg/L 6-BA＋ 0.05 mg/L NAA为生根培养基,研究外植体的芽诱导和生根情况.[结果]外植体消毒的最佳方式为75％酒精30 s＋0.2％ HgCl 6～7 min；试验外植体的污染率、诱导率、死亡率、成活率、生根率分别为14.3％、57.1％、28.6％、50.0％、33.9％.[结论]该研究为独花兰的规模化生产提供了技术指导.
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| 关 键 词： | 独兰花 组织培养 假鳞茎 快速繁殖 |
Study on Tissue Culture of Changnienia amoena |
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| Affiliation: | LIU Guo-shun et al (Xinyang Forestry Science Institute, Xinyang, Henan 464031 ) |
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| Abstract: | [ Objective ] The aim was to study the tissue culture method of Changnienia amoena. [ Method ] Using the pseudobulb of C. amoe- na as explant, the 1/2MS ＋0.5 mg/L 6-BA ＋ 0.05 mg/L NAA as bud induction medium and the 1/2MS ＋ 1.5 mg/L 6-BA ＋ 0.05 mg/L NAA as rooting medium, the conditions of bud induction, and rooting of the explants were studied. [ Result] The best sterilized method of the explants was 75% alcohol for 30 s and O. 2% HgC1 6 - 7 min ; the contamination rate, induction rate, mortality rate, survival rate and rooting rate of the explants were 14.3% , 57.1% , 28.6%, 50% , 33.9% respectively. [ Conclusion] The study provided technical guidance for the large - scale production of C. amoena. |
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| Keywords: | Changnienia amoena Tissue culture Pseudobulb Rapid propagation |
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