| 太空诱变玉米核雄性不育材料的cDNA-AFLP分析 |
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| 引用本文： | 张琳碧,荣廷昭,潘光堂,曹墨菊.太空诱变玉米核雄性不育材料的cDNA-AFLP分析[J].核农学报,2009,23(1):37-41. |
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| 作者姓名： | 张琳碧 荣廷昭 潘光堂 曹墨菊 |
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| 作者单位： | 四川农业大学玉米研究所/作物基因资源与遗传改良教育部重点实验室,四川,雅安,625014 |
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| 基金项目： | 教育部长江学者和创新团队发展计划，国家科技支撑计划 |
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| 摘 要： | 利用cDNA-AFLP技术对太空诱变玉米核雄性不育材料的花药进行差异表达分析，并对差异片段进行回收、克隆和测序，结合半定量RT-PCR方法，分析差异片段在可育株和不育株的表达情况。利用16对引物共回收得到9个差异序列，其中2个为来自不育株的特有片段，7个为来自可育株的特有片段。序列分析发现，来自不育株的 2个EST序列未检测到同源性较高的序列。来自可育株的4个EST序列分别与水稻上推断的查尔酮合成酶、脂酰CoA脱氢酶、蛋白激酶PK12、甘氨酸脱羧酶具有较高的同源性。这些物质可能参与小孢子发育过程中的物质代谢、能量代谢及信号传导等。半定量RT-PCR分析表明，与查尔酮合成酶同源性较高的Z3片断在可育花药发育的早期有较高的表达。而与甘氨酸脱羧酶同源性较高的片断Z8在可育花药发育的中间阶段呈增量表达。这可能与花药发育过程有较高的能量和物质需求有关。
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| 关 键 词： | 玉米 太空诱变 雄性不育 cDNA-AFLP |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
Differential Expression Analysis of Genic Male Sterility by cDNA-AFLP in Maize |
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| ZHANG Lin-bi,RONG Ting-zhao,PAN Guang-tang,CAO Mo-ju.Differential Expression Analysis of Genic Male Sterility by cDNA-AFLP in Maize[J].Acta Agriculturae Nucleatae Sinica,2009,23(1):37-41. |
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| Authors: | ZHANG Lin-bi RONG Ting-zhao PAN Guang-tang CAO Mo-ju |
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| Institution: | Maize Research Institute/Key Laboratory of Crop Genetic Resource and Improvement;Ministry of Education;Sichuan Agricultural University;Ya'an;Sichuan 625014 |
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| Abstract: | The differential expression of male sterility induced by space flight with male fertility was studied using cDNA-AFLP technology.Total RNA was isolated from anther of male sterility and male fertility.Nine differential expression cDNA fragments were obtained with 16 primer combinations.The differential cDNA fragments were eluted,cloned and sequenced.Then half-quantitative RT-PCR was used to study the differential expressions of 4 development stages between sterility and fertility.Sequencing analysis shown 2... |
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| Keywords: | cDNA-AFLP |
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