唐古特白刺组织培养及其培养基筛选研究

 本试验选用唐古特白刺幼嫩茎段和叶片作为材料，研究白刺不同外植体的离体培养技术及其适宜的培养基。结果表明，白刺带芽嫩茎是诱导丛生芽的良好外植体，恒温２０℃以下，可有效降低白刺丛生芽初代培养污染严重的程度；叶片是诱导愈伤组织的良好外植体，且低浓度生长素２，４-Ｄ 培养基较高浓度培养基形成的白刺愈伤组织致密，也不易褐化。白刺的最适增殖、壮芽培养基为ＭＳ＋６-ＢＡ２ｍｇ／Ｌ＋ＮＡＡ１．００ ｍｇ／Ｌ，而且是以腋芽形成丛生芽的方式进行增殖的；最适生根培养基是１／２ ＭＳ＋ＫＴ１ ｍｇ／Ｌ＋ＩＢＡ０．５ ｍｇ／L；形成愈伤组织较好的培养基是ＭＳ＋２，４-Ｄ０．５～１．０ｍｇ／Ｌ。

Research on tissue culture and medium of Nitraria tangutorum

Young stem segments and leaves of Nitraria tangutorum were selected to investigate suitable media and plant regeneration techniques for explants. Immature stems were a good source for explants of tufted shoots, and cultivation at 20℃ can reduce contamination rates of tufted shoots. Leaves were good explants of callus induction as well and a lower concentration of 2,4-D was better for callus than a higher concentration. The optimum multiplication medium for N. tangutorum was MS+6-BA 2 mg/L+NAA 1.00 mg/L and tufted shoots formed from axil buds were the main multiplication method. The optimum medium for root formation was 1/2 MS+KT 1 mg/L+IBA 0.5 mg/L and the optimum medium for callus induction was MS+2.4-D 0.5-1.0 mg/L.