| 牛结核杆菌MPB64蛋白的真核表达 |
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| 引用本文： | 宫强,曲宁,刘思国. 牛结核杆菌MPB64蛋白的真核表达[J]. 华北农学报, 2009, 24(5) |
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| 作者姓名： | 宫强 曲宁 刘思国 |
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| 作者单位： | 河南科技大学,食品与生物工程学院,河南,洛阳,471003;中国农业科学院,哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家科技攻关计划项目 |
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| 摘 要： | 利用PCR技术扩增出牛结核杆菌mpb64基因片段,克隆到真核载体pcDNA3.1(+)上,构建重组质粒pCM64,将该重组质粒转染SP2/0细胞,间接免疫荧光试验和Western blotting检测目的基因的表达情况.结果表明,在转染了重组质粒pCM64的SP2/0细胞中出现绿色荧光,转染的SP2/0细胞泳道23 kDa处出现特异条带,说明mpb64基因在SP2/0细胞中成功进行了瞬时表达.
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| 关 键 词： | 牛结核杆菌 mpb64基因 表达 |
Expression of MPB64 Protein from Mycobacterium bovis in Eukaryotic System |
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| GONG Qiang,QU Ning,LIU Si-guo. Expression of MPB64 Protein from Mycobacterium bovis in Eukaryotic System[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(5) |
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| Authors: | GONG Qiang QU Ning LIU Si-guo |
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| Abstract: | The mpb64 gene fragment amplified by PCR from Mycobacterium bovis was cloned into the eukaryotic ex-pression vector pcDNA3.1 ( + ) , and the recombinant plasmid pCM64 was obtained. Then the recombinant plasmid was transfected into SP2/0 cells in vitro. The expression of target gene was detected by indirect immunofluorescence and Western blotting. The result showed that the green fluorescence appeared in the SP2/0 cells transfected by pCM64 and the specific band about 23 kDa appeared in the electophoretogram of SP2/0 cells, indicaing that the target gene mpb64 had been successfully expressed. |
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| Keywords: | Mycobacterium bovis mpb64 gene Expression |
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