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| 实时定量荧光PCR法检测马铃薯黑胫病菌 | |
| 引用本文： | 杨松,胡林双,吕文河,魏琪,董学志,吕明,高秀妍.实时定量荧光PCR法检测马铃薯黑胫病菌[J].中国马铃薯,2009,23(1). |
| 作者姓名： | 杨松 胡林双 吕文河 魏琪 董学志 吕明 高秀妍 |
| 作者单位： | 1. 东北农业大学农学院,哈尔滨,黑龙江,150030;黑龙江省农业科学院植物脱毒苗木研究所,哈尔滨,黑龙江,150086 2. 黑龙江省农业科学院植物脱毒苗木研究所,哈尔滨,黑龙江,150086 3. 东北农业大学农学院,哈尔滨,黑龙江,150030 4. 黑龙江生态工程职业学院,哈尔滨,黑龙江,150025 |
| 基金项目： | 黑龙江省青年基金，黑龙江省攻关计划，黑龙江省重点攻关计划 |
| 摘 要： | 马铃薯黑胫病是国内外马铃薯产区发生比较普遍的一种细菌性病害,严重地影响了马铃薯的产量和质量。本研究根据Potato Black Leg序列,设计出马铃薯黑胫病菌特异性的引物,对10种供试菌株进行了实时荧光PCR检测。结果表明,该方法的特异性好,可以将马铃薯黑胫病菌和其它马铃薯常见病害相区分;灵敏度较高,可检测出最低的黑胫病菌浓度为3.6~3.9 cfu.mL-1;而且检测时间仅用4 h,大大缩短了检测周期。该方法可有效地应用于马铃薯黑胫病菌的检测和监控。 |
| 关 键 词： | 马铃薯 黑胫病 实时定量荧光PCR |
Detection of Potato Blackleg by Real-time Quantitative PCR |
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| Yang Song,Hu Linshuang,Lu Wenhe,Wei Qi,Dong Xuezhi,Lu Ming,Gao Xiuyan.Detection of Potato Blackleg by Real-time Quantitative PCR[J].Chinese Potato,2009,23(1). | |
| Authors: | Yang Song Hu Linshuang Lu Wenhe Wei Qi Dong Xuezhi Lu Ming Gao Xiuyan |
| Institution: | 1.Northeast Agricultural University;Harbin;Heilongjiang 150030;China;2.Virus-free Seedling Research Institute of Heilongjiang Academy of Agricultural Sciences;Heilongjiang 150086;3.Heilongjiang Vocational Institute of Ecological Engineering;Heilongjiang 150025;China |
| Abstract: | Potato blackleg,a seed tuber-born disease,is caused by Erwinia carotovora subsp.atroseptica(Eca) in temperate regions,which seriously threatens the potato production and tuber quality.In this research,specific primers were designed and developed for potato blackleg based on its sequences,and ten samples were tested by QPCR.The method was specific for potato blackleg and could distinguish this disease from others.Also,it was sensitive and as low as 3.6~3.9 cfu.mL-1 could be detected.The test process needed o... |
| Keywords: | potato potato blackleg real-time quantitative PCR |
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